Overview

  • Product name

    Anti-CDw60 antibody [UM4D4]
  • Description

    Mouse monoclonal [UM4D4] to CDw60
  • Host species

    Mouse
  • Specificity

    ab190716 reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w.
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CDw60. Human rheumatoid synovial T cell line (ST-1).

  • Positive control

    • HL60, Jurkat, MG63, HUT78, K562, YT, U937, HepG2 cells. Tonsil
  • General notes

    Concentration: 200 µg/mL

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 0.05% BSA

    10mM PBS
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    UM4D4
  • Isotype

    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/400.

(for 2 hours at RT)

Flow Cyt Use 5-10µl for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

IHC-Fr 1/100 - 1/200.

(2 hours at RT)

ICC/IF 1/50 - 1/100.

Target

  • Relevance

    CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the costimulation of T-cells.
  • Cellular localization

    Cell Membrane
  • Alternative names

    • 9 O acetyl GD3 antibody
    • 9Oacetyl GD3 antibody
    • CD60 antibody
    • CDw60 antigen antibody

References

ab190716 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab190716.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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