Key features and details
- Mouse monoclonal [LN-1] to CDw75
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgM
Product nameAnti-CDw75 antibody [LN-1]
See all CDw75 primary antibodies
DescriptionMouse monoclonal [LN-1] to CDw75
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to CDw75. (Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes).
- IHC-P: Human spleen tissue. Flow Cytometry: Human lymphocytes.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
Purification notesPurified from bioreactor concentrate by PEG precipitation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab233965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5-1µg for 106 cells.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Incubate with primary antibody for 30 minutes at RT.
RelevanceCDw75 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.
Cellular localizationGolgi Apparatus; Golgi stack; Golgi stack membrane; single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi. Secreted protein; body fluid.
- Alpha 2 6 ST antibody
- B cell antigen CD75 antibody
- Beta galactoside alpha 2 6 sialyltransferase antibody
Formalin-fixed, paraffin-embedded human spleen tissue stained for CDw75 using ab233965 at 1 µg/ml in immunohistochemical analysis.
Flow cytometric analysis of human lymphocytes labeling CDw75 with ab233965 at 1 µg/106 cells (red), compared to an isotype control (green). PPI negative population analyzed. Surface staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233965 has not yet been referenced specifically in any publications.