Recombinant Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4819(2)] to Cdx1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdx1 antibody [EPR4819(2)]
See all Cdx1 primary antibodies -
Description
Rabbit monoclonal [EPR4819(2)] to Cdx1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cdx1. The exact sequence is proprietary.
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Positive control
- Caco-2 and Human stomach lysates; Caco-2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4819(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
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ICC/IF | (1) |
1/100 - 1/250.
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Notes |
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
ICC/IF
1/100 - 1/250. |
Target
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Function
Could play a role in the terminal differentiation of the intestine. -
Tissue specificity
Intestinal epithelium. -
Sequence similarities
Belongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1044 Human
- Omim: 600746 Human
- SwissProt: P47902 Human
- Unigene: 1545 Human
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Alternative names
- Caudal type homeo box transcription factor 1 antibody
- Caudal type homeobox 1 antibody
- Caudal type homeobox protein antibody
see all
Images
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All lanes : Anti-Cdx1 antibody [EPR4819(2)] (ab126748) at 1/1000 dilution
Lane 1 : Caco-2 lysate
Lane 2 : Human stomach lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
ab126748, at 1/100 dilution, staining Cdx1 in Caco-2 cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab126748 has been referenced in 3 publications.
- Meijer BJ et al. Endoplasmic reticulum stress regulates the intestinal stem cell state through CtBP2. Sci Rep 11:9892 (2021). PubMed: 33972635
- Lei D et al. MiR-24 Promotes Cell Growth in Human Glioma by CDX1/PI3K/Akt Signaling Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32876500
- Lv S et al. The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage. Mol Ther Nucleic Acids 14:377-387 (2019). PubMed: 30710891