Recombinant Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-Cdx1 antibody [EPR4819(2)]
See all Cdx1 primary antibodies -
Description
Rabbit monoclonal [EPR4819(2)] to Cdx1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cdx1. The exact sequence is proprietary.
-
Positive control
- Caco-2 and Human stomach lysates; Caco-2 cells
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4819(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). | |
| ICC/IF | 1/100 - 1/250. |
Target
-
Function
Could play a role in the terminal differentiation of the intestine. -
Tissue specificity
Intestinal epithelium. -
Sequence similarities
Belongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1044 Human
- Omim: 600746 Human
- SwissProt: P47902 Human
- Unigene: 1545 Human
-
Alternative names
- Caudal type homeo box transcription factor 1 antibody
- Caudal type homeobox 1 antibody
- Caudal type homeobox protein antibody
see all
Images
-
![Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)](https://www.abcam.com/ps/products/126/ab126748/Images/ab126748-140748-anti-cdx1-antibody-epr48192-western-blot.jpg)
Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)All lanes : Anti-Cdx1 antibody [EPR4819(2)] (ab126748) at 1/1000 dilution
Lane 1 : Caco-2 lysate
Lane 2 : Human stomach lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)](https://www.abcam.com/ps/products/126/ab126748/Images/ab126748-140747-anti-cdx1-antibody-epr48192-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)ab126748, at 1/100 dilution, staining Cdx1 in Caco-2 cells by Immunofluorescence.
Datasheets and documents
References
This product has been referenced in:
- Lv S et al. The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage. Mol Ther Nucleic Acids 14:377-387 (2019). Read more (PubMed: 30710891) »
