Recombinant
RabMAb

Anti-Cdx1 antibody [EPR4819(2)] (ab126748)

Abreviews (1) Submit a Question

Overview

  • Product name
    Anti-Cdx1 antibody [EPR4819(2)]
    See all Cdx1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4819(2)] to Cdx1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cdx1. The exact sequence is proprietary.

  • Positive control
    • Caco-2 and Human stomach lysates; Caco-2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function
      Could play a role in the terminal differentiation of the intestine.
    • Tissue specificity
      Intestinal epithelium.
    • Sequence similarities
      Belongs to the Caudal homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      • Alternative names
        • Caudal type homeo box transcription factor 1 antibody
        • Caudal type homeobox 1 antibody
        • Caudal type homeobox protein antibody
        • Caudal type homeobox protein CDX1 antibody
        • Caudal type homeobox transcription factor 1 antibody
        • Caudal-type homeobox protein 1 antibody
        • CDX1 antibody
        • CDX1_HUMAN antibody
        • Homeobox protein CDX 1 antibody
        • Homeobox protein CDX-1 antibody
        • MGC116915 antibody
        • OTTHUMP00000160524 antibody
        see all

      Images

      • Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        All lanes : Anti-Cdx1 antibody [EPR4819(2)] (ab126748) at 1/1000 dilution

        Lane 1 : Caco-2 lysate
        Lane 2 : Human stomach lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 28 kDa
        Observed band size: 30 kDa (why is the actual band size different from the predicted?)

      • Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        ab126748, at 1/100 dilution, staining Cdx1 in Caco-2 cells by Immunofluorescence.

      References

      ab126748 has not yet been referenced specifically in any publications.

      Publishing research using ab126748? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1 Review or Q&A

      10
      Votes: Highest First

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cdx1 antibody [EPR4819(2)]

       Good
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Differentiated human embryonic stem cells)
      Permeabilization
      Yes - PBS/0.1% Triton X
      Specification
      Differentiated human embryonic stem cells
      Blocking step
      1% BSA/3% Donkey serum in PBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Paraformaldehyde
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      Verified customer

      Submitted Jun 07 2017

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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