Recombinant Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
![Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)](https://www.abcam.com/ps/products/126/ab126748/Images/ab126748-140748-anti-cdx1-antibody-epr48192-western-blot.jpg "Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4819(2)] to Cdx1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Cdx1 antibody [EPR4819(2)]
See all Cdx1 primary antibodies -
Description
Rabbit monoclonal [EPR4819(2)] to Cdx1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cdx1. The exact sequence is proprietary.
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Positive control
- Caco-2 and Human stomach lysates; Caco-2 cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4819(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). | |
| ICC/IF | 1/100 - 1/250. |
Target
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Function
Could play a role in the terminal differentiation of the intestine. -
Tissue specificity
Intestinal epithelium. -
Sequence similarities
Belongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1044 Human
- Omim: 600746 Human
- SwissProt: P47902 Human
- Unigene: 1545 Human
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Alternative names
- Caudal type homeo box transcription factor 1 antibody
- Caudal type homeobox 1 antibody
- Caudal type homeobox protein antibody
see all
Images
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Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)All lanes : Anti-Cdx1 antibody [EPR4819(2)] (ab126748) at 1/1000 dilution
Lane 1 : Caco-2 lysate
Lane 2 : Human stomach lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)](https://www.abcam.com/ps/products/126/ab126748/Images/ab126748-140747-anti-cdx1-antibody-epr48192-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)ab126748, at 1/100 dilution, staining Cdx1 in Caco-2 cells by Immunofluorescence. -
![Anti-Cdx1 antibody [EPR4819(2)] (ab126748)](https://www.abcam.com/ps/products/126/ab126748/Images/ab126748-3-benefits-of-recombinant-antibodies.png)
Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
Protocols
References (1)
ab126748 has been referenced in 1 publication.
- Lv S et al. The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage. Mol Ther Nucleic Acids 14:377-387 (2019). PubMed: 30710891
