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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families Other
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RecombinantRabMAb

Recombinant Anti-Cdx1 antibody [EPR4819(2)] (ab126748)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
  • Anti-Cdx1 antibody [EPR4819(2)] (ab126748)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4819(2)] to Cdx1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Cdx1 antibody [EPR4819(2)]
    See all Cdx1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4819(2)] to Cdx1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cdx1. The exact sequence is proprietary.

  • Positive control

    • Caco-2 and Human stomach lysates; Caco-2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4819(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other

Associated products

  • Alternative Versions

    • Anti-Cdx1 antibody [EPR4819(2)] - BSA and Azide free (ab248164)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Caco 2 whole cell lysate (ab3950)

Applications

Our Abpromise guarantee covers the use of ab126748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Could play a role in the terminal differentiation of the intestine.
    • Tissue specificity

      Intestinal epithelium.
    • Sequence similarities

      Belongs to the Caudal homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P47902 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1044 Human
      • Omim: 600746 Human
      • SwissProt: P47902 Human
      • Unigene: 1545 Human
      • Alternative names

        • Caudal type homeo box transcription factor 1 antibody
        • Caudal type homeobox 1 antibody
        • Caudal type homeobox protein antibody
        • Caudal type homeobox protein CDX1 antibody
        • Caudal type homeobox transcription factor 1 antibody
        • Caudal-type homeobox protein 1 antibody
        • CDX1 antibody
        • CDX1_HUMAN antibody
        • Homeobox protein CDX 1 antibody
        • Homeobox protein CDX-1 antibody
        • MGC116915 antibody
        • OTTHUMP00000160524 antibody
        see all

      Images

      • Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        All lanes : Anti-Cdx1 antibody [EPR4819(2)] (ab126748) at 1/1000 dilution

        Lane 1 : Caco-2 lysate
        Lane 2 : Human stomach lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 28 kDa
        Observed band size: 30 kDa
        why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        ab126748, at 1/100 dilution, staining Cdx1 in Caco-2 cells by Immunofluorescence.
      • Anti-Cdx1 antibody [EPR4819(2)] (ab126748)
        Anti-Cdx1 antibody [EPR4819(2)] (ab126748)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab126748? Please let us know so that we can cite the reference in this datasheet.

      ab126748 has been referenced in 1 publication.

      • Lv S  et al. The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage. Mol Ther Nucleic Acids 14:377-387 (2019). PubMed: 30710891

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cdx1 antibody [EPR4819(2)]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Differentiated human embryonic stem cells)
      Permeabilization
      Yes - PBS/0.1% Triton X
      Specification
      Differentiated human embryonic stem cells
      Blocking step
      1% BSA/3% Donkey serum in PBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 07 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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