Anti-CDX2 antibody [3G9B9] (ab181488)

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Flow Cytometry - Anti-CDX2 antibody [3G9B9] (ab181488)
  • Western blot - Anti-CDX2 antibody [3G9B9] (ab181488)
  • Western blot - Anti-CDX2 antibody [3G9B9] (ab181488)

Key features and details

  • Mouse monoclonal [3G9B9] to CDX2
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-CDX2 antibody [3G9B9]
    See all CDX2 primary antibodies

  • Description

    Mouse monoclonal [3G9B9] to CDX2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CDX2 aa 176-303. (Expressed in E.coli).
    Sequence:

    SLGSQVKTRTKDKYRVVYTDHQRLELEKEFHYSRYITIRRKAELAATLGL SERQVKIWFQNRRAKERKINKKKLQQQQQQQPPQPPPPPPQPPQPQPGPL RSVPEPLSPVSSLQASVPGS VPGVLGPT


    Database link: Q99626
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human CDX2 (AA: 176-303) recombinant protein; CDX2 (AA: 176-303)-hIgGFc transfected HEK293 cell lysate; HeLa cells.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Recombinant fragment
Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 33 kDa.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 33 kDa.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

  • Sequence similarities

    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylation of Ser-60 mediates the transactivation capacity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Caudal type homeo box 2 antibody
      • Caudal type homeo box transcription factor 2 antibody
      • Caudal type homeobox 2 antibody
      • Caudal type homeobox protein 2 antibody
      • Caudal type homeobox transcription factor 2 antibody
      • Caudal-type homeobox protein 2 antibody
      • CDX 2 antibody
      • CDX 3 antibody
      • CDX-3 antibody
      • Cdx2 antibody
      • CDX2/AS antibody
      • CDX2_HUMAN antibody
      • CDX3 antibody
      • Homeobox protein CDX 2 antibody
      • Homeobox protein CDX-2 antibody
      see all

    Images

    • Flow Cytometry - Anti-CDX2 antibody [3G9B9] (ab181488)
      Flow Cytometry - Anti-CDX2 antibody [3G9B9] (ab181488)

      Flow cytometric analysis of Hela cells labeling CDX2 with ab181488 at 1/200 dilution (green) compared to a negative control (red).

    • Western blot - Anti-CDX2 antibody [3G9B9] (ab181488)
      Western blot - Anti-CDX2 antibody [3G9B9] (ab181488)
      All lanes : Anti-CDX2 antibody [3G9B9] (ab181488) at 1/500 dilution

      Lane 1 : HEK293 cell lysate
      Lane 2 : CDX2 (AA: 176-303)-hIgGFc transfected HEK293 cell lysate

      Predicted band size: 33 kDa

    • Western blot - Anti-CDX2 antibody [3G9B9] (ab181488)
      Western blot - Anti-CDX2 antibody [3G9B9] (ab181488)
      Anti-CDX2 antibody [3G9B9] (ab181488) at 1/500 dilution + CDX2 (AA: 176-303) recombinant protein.

      Predicted band size: 33 kDa



      Expected MWt is 40.1 kDa.

    References (0)

    Publishing research using ab181488? Please let us know so that we can cite the reference in this datasheet.

    ab181488 has not yet been referenced specifically in any publications.

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