Key features and details
- Rabbit polyclonal to CDX2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDX2 antibody
See all CDX2 primary antibodies
DescriptionRabbit polyclonal to CDX2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment within Human CDX2 (internal sequence). The exact sequence is proprietary.
Database link: Q99626
- WB: H1299 whole cell lysate. IHC-P: Human colon tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Sequence similaritiesBelongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain.
modificationsPhosphorylation of Ser-60 mediates the transactivation capacity.
- Information by UniProt
- Caudal type homeo box 2 antibody
- Caudal type homeo box transcription factor 2 antibody
- Caudal type homeobox 2 antibody
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDX2 (green) using ab227201 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Anti-CDX2 antibody (ab227201) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 33 kDa
12% SDS PAGE
Paraffin-embedded human colon tissue stained for CDX2 with ab227201 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227201 has not yet been referenced specifically in any publications.