Key features and details
- Goat polyclonal to CDX2
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDX2 antibody
See all CDX2 primary antibodies
DescriptionGoat polyclonal to CDX2
SpecificityNo crossreactivity expected with CDX1 or CDX4.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Hamster
- Purchase matching WB positive control:Recombinant Human CDX2 protein
- WB: Human colon lysate; IHC-P: Human colon tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab48009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Sequence similaritiesBelongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain.
modificationsPhosphorylation of Ser-60 mediates the transactivation capacity.
- Information by UniProt
- Caudal type homeo box 2 antibody
- Caudal type homeo box transcription factor 2 antibody
- Caudal type homeobox 2 antibody
Immunohistochemical analysis of paraffin-embedded hukan colon tissue staing CDX2 with ab48009 at 5µg/ml, followed by AP staining. Steamed antigen retrieval with citrate buffer pH 6.
Anti-CDX2 antibody (ab48009) at 1 µg/ml + Human Colon lysate (35µg protein in RIPA buffer).
Predicted band size: 34 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. The observed 35kDa band was successfully blocked by incubation with the immunizing peptide.
ab48009 has been referenced in 1 publication.
- Liu X et al. A novel case of endometrial dedifferentiated adenocarcinoma associated with MLH1 promotor hypermethylation and microsatellite instability. Pathol Res Pract 214:1904-1908 (2018). PubMed: 30173944