Our Abpromise guarantee covers the use of ab48009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • Function

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • Sequence similarities

    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational

    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2/AS antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all


  • Immunohistochemical analysis of paraffin-embedded hukan colon tissue staing CDX2 with ab48009 at 5µg/ml, followed by AP staining. Steamed antigen retrieval with citrate buffer pH 6.

  • Anti-CDX2 antibody (ab48009) at 1 µg/ml + Human Colon lysate (35µg protein in RIPA buffer).

    Predicted band size: 34 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence. Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. The observed 35kDa band was successfully blocked by incubation with the immunizing peptide.


This product has been referenced in:

  • Liu X  et al. A novel case of endometrial dedifferentiated adenocarcinoma associated with MLH1 promotor hypermethylation and microsatellite instability. Pathol Res Pract 214:1904-1908 (2018). Read more (PubMed: 30173944) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x Dako Target Retrival Solution in Water
Yes - 0.5% Tween-20 in PBS
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 22 2017


Thank you for your call today. Please see below for information about our testing discount program. Please let me know if you have any questions or if there is anything else that we can do for you!

Since ab48009 has not been testedwith mouse samples,I can offer a discount off a future purchase if you buy ab48009 now, test itwith mouse samples,and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab48009in mouse. I will then send a discount code.

2. Purchase ab48009 either by phone, fax, or online (www.abcam.com).

3. Test it with mouse samples.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab48009 turns out to be unsuitable for use with mouse samples, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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