Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab48009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • Sequence similarities

    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2/AS antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded hukan colon tissue staing CDX2 with ab48009 at 5µg/ml, followed by AP staining. Steamed antigen retrieval with citrate buffer pH 6.

  • Anti-CDX2 antibody (ab48009) at 1 µg/ml + Human Colon lysate (35µg protein in RIPA buffer).

    Predicted band size: 34 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence. Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. The observed 35kDa band was successfully blocked by incubation with the immunizing peptide.

References

This product has been referenced in:

  • Liu X  et al. A novel case of endometrial dedifferentiated adenocarcinoma associated with MLH1 promotor hypermethylation and microsatellite instability. Pathol Res Pract 214:1904-1908 (2018). Read more (PubMed: 30173944) »
See 1 Publication for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x Dako Target Retrival Solution in Water
Permeabilization
Yes - 0.5% Tween-20 in PBS
Specification
Colon
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde

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Verified customer

Submitted Jun 22 2017

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