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    cdx2-antibody-sp54-ab101532.pdf

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Stem Cells Lineage Markers Endoderm
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RecombinantRabMAb

Recombinant Anti-CDX2 antibody [SP54] (ab101532)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] (ab101532)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] (ab101532)
  • Flow Cytometry - Anti-CDX2 antibody [SP54] (ab101532)
  • Flow Cytometry - Anti-CDX2 antibody [SP54] (ab101532)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] (ab101532)
  • Anti-CDX2 antibody [SP54] (ab101532)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP54] to CDX2
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

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Protein
Product image
Recombinant Human CDX2 protein (ab114247)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CDX2 antibody [SP54]
    See all CDX2 primary antibodies
  • Description

    Rabbit monoclonal [SP54] to CDX2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CDX2 aa 100-200 (N terminal). The exact sequence is proprietary.
    Database link: Q99626

  • Positive control

    • IHC-P: Human colon carcinoma tissue. Flow Cyt: HT-29 and Caco-2 cells ICC/IF: Caco-2 cells
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP54
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Trophectoderm

Associated products

  • Alternative Versions

    • Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CDX2 protein (ab114247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab101532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
All applications
Mouse
Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow Cyt
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF
1/50.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow Cyt
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF
1/50.

Target

  • Function

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • Sequence similarities

    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q99626 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1045 Human
    • Entrez Gene: 12591 Mouse
    • Omim: 600297 Human
    • SwissProt: Q99626 Human
    • SwissProt: P43241 Mouse
    • Unigene: 174249 Human
    • Unigene: 20358 Mouse
    • Alternative names

      • Caudal type homeo box 2 antibody
      • Caudal type homeo box transcription factor 2 antibody
      • Caudal type homeobox 2 antibody
      • Caudal type homeobox protein 2 antibody
      • Caudal type homeobox transcription factor 2 antibody
      • Caudal-type homeobox protein 2 antibody
      • CDX 2 antibody
      • CDX 3 antibody
      • CDX-3 antibody
      • Cdx2 antibody
      • CDX2/AS antibody
      • CDX2_HUMAN antibody
      • CDX3 antibody
      • Homeobox protein CDX 2 antibody
      • Homeobox protein CDX-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] (ab101532)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] (ab101532)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CDX2 with ab101532 at 1/100 dilution (1.11 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab101532 for 30 mins at room temperature.

    • Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] (ab101532)
      Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] (ab101532)

      Immunocytochemistry/ Immunofluorescence analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) cells labeling CDX2 with purified ab101532 at 1/50 (2.22 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry - Anti-CDX2 antibody [SP54] (ab101532)
      Flow Cytometry - Anti-CDX2 antibody [SP54] (ab101532)

      Flow cytometry analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) labeling CDX2 with purified ab101532 at 1/200 dilution (0.56 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (black). Unlableled control -Unlabelled cells (blue).

    • Flow Cytometry - Anti-CDX2 antibody [SP54] (ab101532)
      Flow Cytometry - Anti-CDX2 antibody [SP54] (ab101532)

      Flow cytometric analysis of rabbit anti-CDX2 (SP54) antibody ab101532 (1/100) in HT-29 cells (green) compared to negative control of rabbit IgG (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] (ab101532)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] (ab101532)
      Staining of Human CDX2 in a Formalin/PFA-fixed paraffin-embedded section of Human Colon Carcinoma using ab101532 at a dilution of 1/100.
    • Anti-CDX2 antibody [SP54] (ab101532)
      Anti-CDX2 antibody [SP54] (ab101532)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab101532? Please let us know so that we can cite the reference in this datasheet.

    ab101532 has been referenced in 1 publication.

    • Chen Y  et al. 5-Aza-dC treatment induces mesenchymal-to-epithelial transition in 1st trimester trophoblast cell line HTR8/SVneo. Biochem Biophys Res Commun 432:116-22 (2013). ICC/IF ; Human . PubMed: 23376068

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Please use the links above to contact us or submit feedback about this product.

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    For licensing inquiries, please contact partnerships@abcam.com

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