Recombinant
RabMAb

Recombinant Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)

Overview

  • Product name

    Anti-CDX2 antibody [SP54] - BSA and Azide free
    See all CDX2 primary antibodies
  • Description

    Rabbit monoclonal [SP54] to CDX2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human CDX2 aa 100-200 (N terminal). The exact sequence is proprietary.
    Database link: Q99626

  • Positive control

    • IHC-P: Human colon carcinoma tissue. Flow Cyt: HT-29 cells.
  • General notes

    Ab240936 is the carrier-free version of ab101532. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240936 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab240936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

 

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • Sequence similarities

    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2/AS antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CDX2 with ab101532 at 1/100 dilution (1.11 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101532 for 30 mins at room temperature.

    This image was generated using ab101532, the same clone, but with a different buffer formulation.

  • Staining of CDX2 in a Formalin/PFA-fixed paraffin-embedded section of human colon carcinoma tissue using ab101532 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab101532)

  • Flow cytometric analysis of rabbit anti-CDX2 (SP54) antibody ab101532(1/100) in HT29 cells (green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab101532)

References

ab240936 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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