Product nameAnti-CDYL antibody - ChIP Grade
See all CDYL primary antibodies
DescriptionRabbit polyclonal to CDYL - ChIP Grade
Tested applicationsSuitable for: CHIPseq, ChIP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide derived from residues 300 - 400 of Mouse CDYL.
- This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab5188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|CHIPseq||Use at an assay dependent concentration. PubMed: 22009739|
|ChIP||Use at an assay dependent concentration. PubMed: 22009739|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).|
FunctionActs as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4.
Tissue specificityUbiquitous. Expressed at moderate levels in all tissues examined.
Sequence similaritiesContains 1 chromo domain.
- Information by UniProt
- CDY-like antibody
- CDY-like autosomal antibody
- Cdyl antibody
All lanes : Anti-CDYL antibody - ChIP Grade (ab5188) at 1/1000 dilution
Lane 1 : COS7 (monkey) cells
Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid
Predicted band size: 66 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
Rabbit polyclonal to CDYL (ab5188) at 1/1000.
Left lane (-) COS7 (monkey) cells.
Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.
Image courtesy of Human Protein Atlas
ab5188 staining CDYL in human male kidney, showing a distinct and strong staining pattern in tubuli and glomeruli cells. Paraffin embedded human skin tissue was incubated with ab5188 (1/1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6..
ab5188 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
All lanes : Anti-CDYL antibody - ChIP Grade (ab5188) at 1 µg/ml
Lane 1 : COS7 Untransfected Whole Cell Lysate
Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
This product has been referenced in:
- Qi C et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . Read more (PubMed: 24671995) »
- Escamilla-Del-Arenal M et al. Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2. Mol Cell Biol 33:5005-20 (2013). WB, ICC/IF . Read more (PubMed: 24144980) »