Overview

  • Product name
    Anti-CDYL antibody - ChIP Grade
    See all CDYL primary antibodies
  • Description
    Rabbit polyclonal to CDYL - ChIP Grade
  • Host species
    Rabbit
  • Tested applications
    Suitable for: CHIPseq, ChIP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from residues 300 - 400 of Mouse CDYL.

    Read Abcam's proprietary immunogen policy .

  • Positive control
    • This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)

Properties

Applications

Our Abpromise guarantee covers the use of ab5188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
CHIPseq Use at an assay dependent concentration. PubMed: 22009739
ChIP Use at an assay dependent concentration. PubMed: 22009739
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).

Target

  • Function
    Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4.
  • Tissue specificity
    Ubiquitous. Expressed at moderate levels in all tissues examined.
  • Sequence similarities
    Contains 1 chromo domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDY-like antibody
    • CDY-like autosomal antibody
    • Cdyl antibody
    • CDYL: chromodomain protein Y-like antibody
    • CDYL1 antibody
    • CDYL1_HUMAN antibody
    • chromodomain protein Y chromosome-like 6 antibody
    • Chromodomain protein Y like antibody
    • Chromodomain Y-like protein antibody
    • DKFZP586C1622 antibody
    • MGC131936 antibody
    • OTTHUMP00000015984 antibody
    • OTTHUMP00000211185 antibody
    • OTTHUMP00000211186 antibody
    • OTTHUMP00000211189 antibody
    • testis-specific chromodomain Y-like protein antibody
    • Weakly similar to enoyl-CoA hydratase [H.sapiens] antibody
    see all

Images

  • All lanes : Anti-CDYL antibody - ChIP Grade (ab5188) at 1/1000 dilution

    Lane 1 : COS7 (monkey) cells
    Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid

    Predicted band size: 66 kDa
    Observed band size: 83 kDa
    why is the actual band size different from the predicted?



    Rabbit polyclonal to CDYL (ab5188) at 1/1000.

    Left lane (-) COS7 (monkey) cells.
    Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.

  • Image courtesy of Human Protein Atlas

    ab5188 staining CDYL in human male kidney, showing a distinct and strong staining pattern in tubuli and glomeruli cells. Paraffin embedded human skin tissue was incubated with ab5188 (1/1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab5188 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • All lanes : Anti-CDYL antibody - ChIP Grade (ab5188) at 1 µg/ml

    Lane 1 : COS7 Untransfected Whole Cell Lysate
    Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?
    Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

References

This product has been referenced in:
  • Qi C  et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . Read more (PubMed: 24671995) »
  • Escamilla-Del-Arenal M  et al. Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2. Mol Cell Biol 33:5005-20 (2013). WB, ICC/IF . Read more (PubMed: 24144980) »
See all 8 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
ChIP
Detection step
Real-time PCR
Sample
Rat Cell lysate - nuclear (Hippocampal primary Neurons)
Specification
Hippocampal primary Neurons
Negative control
normal Rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehide 1%
Positive control
BDNF Promoter II

Abcam user community

Verified customer

Submitted Oct 06 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 25 2014

Answer

Thank you for your reply. Unfortunately, I am not an expert in this field and I have not been able to find any publications relating to the expression profiles in mice stem cells. I am not aware of a particular protein or antibody that would recognise a target uniquely expressed from the Y chromosome in stem cells at least. The majority of genes expressed from the Y chromosome also have X homologs e.g. Smcy and its X homolog Smcx. However, we do have an Sry antibody which is the sex determing factor although I am under the impression that it is uniquely expressed in testes. I would think that the best way to identify male from female cells would be to perform DNA FISH using a commercially available kit and probing X and Y loci. e.g. Males would have green and red foci whereas females would have two green foci. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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Answer

Thank you for your enquiry. The peptide sequence used to raise this antibody was a synthetic peptide conjugated to KLH derived from within residues 301-314 of Mouse CDYL, corresponding to sequence TGVTAGKRKFIDDR. In reponse to your question it will depend on whether you wish to localize male human or mouse stem cells transplanted into female mice. Human CDYL is expressed from the Y chromosome but according to http://www.pnas.org/cgi/content/abstract/99/13/8707, murine CDYL is autosomal. I have also found out that in mice it is expressed as 2 transcripts: a ubiquitous transcript and a highly expressed testis-specific transcript. Therefore if you wish to use it as a marker it will depend on the level of expression in the ubiquitous transcript. Unfortunately I could not determine whether it's expression was sex specific, such details were not present on the EXPASY database. I hope that this information helps. Please do not hesitate to contact me should you require further assistance.

Read More

Answer

Thank you for your enquiry. We would suggest blocking the non-specific binding sited with 5% BSA overnight at 4oC. For further information about Western blot application, please take a look at the Protocol on our website

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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