Anti-CEACAM 5 + 6 antibody [MUS] (ab4539)
Key features and details
- Mouse monoclonal [MUS] to CEACAM 5 + 6
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CEACAM 5 + 6 antibody [MUS] -
Description
Mouse monoclonal [MUS] to CEACAM 5 + 6 -
Host species
Mouse -
Specificity
MUS reacts specifically with CEACAM 5 (CEA/CD66e) and CEACAM 6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry. -
Species reactivity
Reacts with: Human -
Immunogen
Full length native Carcino Embryonic Antigen(partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Schozel S et al.).
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General notes
Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MUS -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Flow Cyt: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution. Predicted molecular weight Carcino Embryonic Antigen: 84 kDa. Predicted molecular weight CEACAM 6: 41 kDa
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 1048 Human
- Entrez Gene: 4680 Human
- Omim: 114890 Human
- Omim: 163980 Human
- SwissProt: P06731 Human
- SwissProt: P40199 Human
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Alternative names
- Carcinoembryonic antigen antibody
- CD66c antibody
- CD66e antibody
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Protocols
Datasheets and documents
References (2)
ab4539 has been referenced in 2 publications.
- Edwards MM et al. Idiopathic preretinal glia in aging and age-related macular degeneration. Exp Eye Res 150:44-61 (2016). PubMed: 26220834
- Tsutsumida H et al. RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells. Clin Cancer Res 12:2976-87 (2006). PubMed: 16707592
