Overview

  • Product name

    Anti-CEACAM 5 + 6 antibody [MUS]
  • Description

    Mouse monoclonal [MUS] to CEACAM 5 + 6
  • Host species

    Mouse
  • Specificity

    MUS reacts specifically with CEACAM 5 (CEA/CD66e) and CEACAM 6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry.
  • Tested applications

    Suitable for: Flow Cyt, ELISA, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native Carcino Embryonic Antigen(partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Schozel S et al.).

  • General notes

    Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    MUS
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ELISA
IHC-Fr
WB
  • Application notes
    ELISA: Use at an assay dependant dilution.
    Flow Cyt: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution. Predicted molecular weight Carcino Embryonic Antigen: 84 kDa. Predicted molecular weight CEACAM 6: 41 kDa

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
    • Cellular localization

      Plasma membrane
    • Database links

    • Alternative names

      • Carcinoembryonic antigen antibody
      • CD66c antibody
      • CD66e antibody
      • CEA antibody
      • CEACAM5 antibody
      • CEACAM6 antibody
      • CEAL antibody
      • NCA antibody
      see all

    References

    This product has been referenced in:

    • Tsutsumida H  et al. RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells. Clin Cancer Res 12:2976-87 (2006). Read more (PubMed: 16707592) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Actually, since both CEA (CEACAM5) and NCA (CEACAM6) are glycosylated proteins, the apparent molecular weight depends on the extraction method you used...: NCA: In case of a PCA extraction, NCA is usually detected at a molecular weight of 55 kDa, whereas with more gentle methods you will be able to detect a 90 kDa band. CEA: Usually, CEA can be stained in a rather smeary way: in-between 150 and 200 kDa you will be able to detect most probably two distinct bands (differing by approx 20 or 30 kDa), however, again, this might depend on you extraction method.

    Read More

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