Overview

  • Product name

    Anti-CEACAM1 + CEACAM5 antibody [C66/261]
    See all CEACAM1 + CEACAM5 primary antibodies
  • Description

    Mouse monoclonal [C66/261] to CEACAM1 + CEACAM5
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IP, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CEACAM1 + CEACAM5. Human colon carcinoma extract
    Database link: P06731

  • Positive control

    • MCF7 or 293T cells. Human colon carcinoma tissue; Stomach lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab190718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5-1µg for 1010-6 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at 1-0.5 µg/mg of lysate.
IHC-Fr Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

  • Relevance

    CEACAM1 and CEACAM5 belong to the carcinoembryonic antigen (CEA) gene family. It is expressed on cells of epithelial and myeloid origin. CEACAM1 expression is downregulated in many tumors indicating a tumor - suppressive function. The antitumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of antiangiogenic molecules from the cells. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM. CEACAM5 is a cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is a homodimer. It is a receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • Cellular localization

    Isoforms A, B, C: Cell membrane; Single-pass type I membrane protein. Isoforms G, H, I: Secreted.
  • Database links

  • Alternative names

    • Antigen CD66e antibody
    • ATP dependent taurocolate carrier protein antibody
    • BGP 1 antibody
    • BGP antibody
    • BGP I antibody
    • BGP1 antibody
    • BGPI antibody
    • Biliary glycoprotein antibody
    • Biliary glycoprotein 1 antibody
    • Biliary glycoprotein adhesion molecule antibody
    • C CAM 105 antibody
    • Carcinoembryonic antigen 1 antibody
    • Carcinoembryonic antigen 7 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 5 antibody
    • CD66 antigen antibody
    • CD66a antigen antibody
    • CD66e antibody
    • CEA antibody
    • CEA related cell adhesion molecule 1 (bone gamma carboxyglutamic acid (Gla) protein) (osteocalcin) antibody
    • CEA related cell adhesion molecule 1 antibody
    • CEACAM 1 antibody
    • DKFZp781M2392 antibody
    • mCEA1 antibody
    • Meconium antigen 100 antibody
    • Osteocalcin antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling CEACAM1 + CEACAM5 with ab190718 at 0.5 μg/ml.

  • Anti-CEACAM1 + CEACAM5 antibody [C66/261] (ab190718) at 0.5 µg/ml + Stomach lysate

    Predicted band size: 77 kDa

References

ab190718 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190718.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up