Recombinant
RabMAb

Recombinant Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

Overview

  • Product name

    Anti-CEACAM1 + CEACAM6 antibody [EPR19878]
    See all CEACAM1+CEACAM6 primary antibodies
  • Description

    Rabbit monoclonal [EPR19878] to CEACAM1 + CEACAM6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CEACAM1 aa 1-450. The exact sequence is proprietary.
    Database link: P13688

  • Positive control

    • WB: Human CEACAM1 recombinant protein; human CEACAM6 recombinant protein; BxPC-3, HepG2, A549 and HT-29 whole cell lysates; human kidney and fetal liver lysates. IHC-P: Human colon and liver cancer tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19878
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Cellular localization

    CEACAM1: Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact. CEACAM6: Cell membrane.
  • Database links

  • Form

    CEACAM1: There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.

Images

  • All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : Human CEACAM1 recombinant protein
    Lane 2 : Human CEACAM5 recombinant protein
    Lane 3 : Human CEACAM6 recombinant protein
    Lane 4 : Human CEACAM7 recombinant protein
    Lane 5 : Human CEACAM8 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human CEACAM1 recombinant protein contains aa35-428 with GST-tag and His-Tag®. Human CEACAM5, CEACAM6, CEACAM7, CEACAM8 recombinant proteins contain aa35-422, aa35-326, aa35-326 and aa35-335 respectively, and all with a His-Tag®.

  • All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
    Lane 2 : Human kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa
    Observed band size: 110-150,75-90 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, PMID: 10751340, PMID: 17081782).

  • All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa
    Observed band size: 110-150 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 minute; Lane 2: 3 seconds; Lane 3: 30 seconds; Lane 4: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, PMID: 10751340, PMID: 17081782).

  • All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    HUVEC does not express CEACAM 6 and PC-3 does not express CEACAM 1 (PMID: 22918079, PMID: 10359532).

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Brush border staining on human colon is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on human liver cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

References

ab206431 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab206431.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up