Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal  to CEACAM1 - BSA and Azide free
- Suitable for: Flow Cyt, ICC
- Reacts with: Mouse
Product nameAnti-CEACAM1 antibody  - BSA and Azide free
See all CEACAM1 primary antibodies
DescriptionRabbit monoclonal  to CEACAM1 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, ICCmore details
Species reactivityReacts with: Mouse
Recombinant fragment corresponding to Mouse CEACAM1 aa 1-428. Genbank: NP_001034274.1
Database link: P31809
- Flow Cyt: Mouse BABL/c splenocytes. ICC: Mouse splenocytes.
Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab277129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/25 - 1/100.|
|ICC||1/20 - 1/100.|
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
- Information by UniProt
FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
Flow cytometric analysis of CEACAM1 expression on mouse BABL/c splenocytes. Cells were stained with ab277129 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also stained with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of CEACAM1 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277129 at 1/60 dilution at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277129 has not yet been referenced specifically in any publications.