Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR032F] to CEACAM1
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Product nameAnti-CEACAM1 antibody [BLR032F]
See all CEACAM1 primary antibodies
DescriptionRabbit monoclonal [BLR032F] to CEACAM1
Tested applicationsSuitable for: WB, ICC, IHC-P, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CEACAM1 (extracellular).
Database link: P13688
This product is sold under License from Bethyl Laboratories, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
- Information by UniProt
FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
CEACAM1 was immunoprecipitated from 1.0 mg UACC-257 whole cell lysate with ab243889 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243889 at 1/1000 dilution.
Lane 1: ab243889 IP in UACC-257 whole cell lysate.
Lane 2: Control IgG in UACC-257 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
Western blot analysis using ab243889 at 1/1000 dilution.
Lane 1: UACC-257 whole cell lysate (5 µg).
Lane 2: SK-MEL-28 whole cell lysate (5 µg).
Lane3: HepG2 whole cell lysate (5 µg).
Lane 4: SUP-M2 whole cell lysate (5 µg).
Lane 5: HeLa whole cell lysate (5 µg).
Lane 6: HEK-293T whole cell lysate (5 µg).
Lane 7: Jurkat whole cell lysate (5 µg).
Lane 8: RAW 264.7 whole cell lysate (5 µg).
Lane 9: TCMK-1 whole cell lysate (5 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 1 second.
Formalin-fixed, paraffin-embedded HT-29 (human colorectal adenocarcinoma cell line) cells labeling CEACAM1 using ab243889 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CEACAM1 using ab243889 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243889 has not yet been referenced specifically in any publications.