Product nameAnti-CEACAM1 antibody [BLR032F]
See all CEACAM1 primary antibodies
DescriptionRabbit monoclonal [BLR032F] to CEACAM1
Tested applicationsSuitable for: WB, ICC, IHC-P, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CEACAM1 (extracellular).
Database link: P13688
This product is sold under License from Bethyl Laboratories, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
- Information by UniProt
FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
CEACAM1 was immunoprecipitated from 1.0 mg UACC-257 whole cell lysate with ab243889 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243889 at 1/1000 dilution.
Lane 1: ab243889 IP in UACC-257 whole cell lysate.
Lane 2: Control IgG in UACC-257 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
Western blot analysis using ab243889 at 1/1000 dilution.
Lane 1: UACC-257 whole cell lysate (5 µg).
Lane 2: SK-MEL-28 whole cell lysate (5 µg).
Lane3: HepG2 whole cell lysate (5 µg).
Lane 4: SUP-M2 whole cell lysate (5 µg).
Lane 5: HeLa whole cell lysate (5 µg).
Lane 6: HEK-293T whole cell lysate (5 µg).
Lane 7: Jurkat whole cell lysate (5 µg).
Lane 8: RAW 264.7 whole cell lysate (5 µg).
Lane 9: TCMK-1 whole cell lysate (5 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 1 second.
Formalin-fixed, paraffin-embedded HT-29 (human colorectal adenocarcinoma cell line) cells labeling CEACAM1 using ab243889 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CEACAM1 using ab243889 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
ab243889 has not yet been referenced specifically in any publications.