Overview

  • Product name

    Anti-CEACAM1 antibody [BLR032F]
    See all CEACAM1 primary antibodies
  • Description

    Rabbit monoclonal [BLR032F] to CEACAM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CEACAM1 (extracellular).
    Database link: P13688

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.2
    Preservative: 0.09% Sodium azide
    Constituents: 98% Borate buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purification notes

    Recombinant antibody was purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    BLR032F
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab243889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC 1/100 - 1/500.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Use 20µl/mg lysate.

Target

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
  • Information by UniProt
  • Database links

  • Form

    There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
  • Alternative names

    • Antigen CD66 antibody
    • BGP 1 antibody
    • BGP antibody
    • BGP-1 antibody
    • BGPI antibody
    • Biliary glycoprotein 1 antibody
    • Biliary glycoprotein adhesion molecule antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 1 antibody
    • CD66a antibody
    • CD66a antigen antibody
    • CEACAM1 antibody
    • CEAM1_HUMAN antibody
    • meconium antigen 100 antibody
    see all

Images

  • CEACAM1 was immunoprecipitated from 1.0 mg UACC-257 whole cell lysate with ab243889 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243889 at 1/1000 dilution.

    Lane 1: ab243889 IP in UACC-257 whole cell lysate.

    Lane 2: Control IgG in UACC-257 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 1 second.

  • Western blot analysis using ab243889 at 1/1000 dilution.

    Lane 1: UACC-257 whole cell lysate (5 µg).

    Lane 2: SK-MEL-28 whole cell lysate (5 µg).

    Lane3: HepG2 whole cell lysate (5 µg).

    Lane 4: SUP-M2 whole cell lysate (5 µg).

    Lane 5: HeLa whole cell lysate (5 µg).

    Lane 6: HEK-293T whole cell lysate (5 µg).

    Lane 7: Jurkat whole cell lysate (5 µg).

    Lane 8: RAW 264.7 whole cell lysate (5 µg).

    Lane 9: TCMK-1 whole cell lysate (5 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 1 second.

  • Formalin-fixed, paraffin-embedded HT-29 (human colorectal adenocarcinoma cell line) cells labeling CEACAM1 using ab243889 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CEACAM1 using ab243889 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

References

ab243889 has not yet been referenced specifically in any publications.

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