Overview

  • Product name
    Anti-CEACAM1 antibody [EPR4048]
    See all CEACAM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4048] to CEACAM1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CEACAM1 aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control
    • HepG2 cell lysate, A549 cell lysate, Human fetal liver tissue lysate, Human kidney tissue, Human liver tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4048
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Sequence similarities
      Belongs to the immunoglobulin superfamily. CEA family.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
    • Information by UniProt
    • Database links
    • Form
      There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
    • Alternative names
      • Antigen CD66 antibody
      • BGP 1 antibody
      • BGP antibody
      • BGP-1 antibody
      • BGPI antibody
      • Biliary glycoprotein 1 antibody
      • Biliary glycoprotein adhesion molecule antibody
      • Biliary glycoprotein antibody
      • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
      • carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody
      • Carcinoembryonic antigen-related cell adhesion molecule 1 antibody
      • CD66a antibody
      • CD66a antigen antibody
      • CEACAM1 antibody
      • CEAM1_HUMAN antibody
      • meconium antigen 100 antibody
      see all

    Images

    • ab108390, at 1/100 dilution, staining CEACAM1 in Human kidney tissue by immunohistochemistry.
    • All lanes : Anti-CEACAM1 antibody [EPR4048] (ab108390) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    • ab108390, at 1/100 dilution, staining CEACAM1 in Human liver tissue by immunohistochemistry.

    References

    This product has been referenced in:
    • Li J  et al. Abnormal expression of circulating and tumor-infiltrating carcinoembryonic antigen-related cell adhesion molecule 1 in patients with glioma. Oncol Lett 15:3496-3503 (2018). Read more (PubMed: 29467871) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us and your interest in our products.

    As discussed over the phone the anti-CEACAM1 antibody (ab108390) is supplied in 100 ul for £190. The lot we currently have in stock has a concentration of 0.1980 mg/ml. Please however bear in mind that rabbit monoclonal antibodies generally have a higher affinity and you therefore tend to need less when using them compared to rabbit polylconal and mouse monoclonal antibodies.

    We are currently running an offer where you can receive a free rabbit monoclonal such as this with any order placed with Abcam. More information on this offer can be found from our main webpage:

    https://www.abcam.com

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

    Read More

    Answer

    Thank you for your inquiry. I was liaising with the laboratories to find out more about our pan-CEACAM- and CEACAM1-antibodies. Unfortunately, it turned out that we do not have any suitable antibody available: The immunogens of our CEACAM1 antibodies share a sequence homology with dog of 67% at the best. Thus, we would not predict a cross-reactivity for one of these antibodies. The same applies for our pan-CEACAM antibodies: The human CEACAM proteins used as immunogens reveal sequence similarities with the dog ortholog that are below 70%, suggesting that a cross-reactivity of the antibody is rather unlikely. If you have not done yet, I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I am sorry that I could not be more helpful on this occasion and hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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