Recombinant Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free (ab179460)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4048] to CEACAM1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free
See all CEACAM1 primary antibodies -
Description
Rabbit monoclonal [EPR4048] to CEACAM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CEACAM1 aa 50-150 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: HepG2 cell lysate, A549 cell lysate, Human fetal liver tissue lysate, Human liver tissue and His-tagged human CEAM-1 fragment. IHC-P: Human kidney tissue, HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag and HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag.
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General notes
ab179460 is the carrier-free version of ab108390.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4048 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. |
Target
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Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact. - Information by UniProt
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Database links
- Entrez Gene: 634 Human
- Omim: 109770 Human
- SwissProt: P13688 Human
- Unigene: 512682 Human
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Form
There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S. -
Alternative names
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
see all
Images
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All lanes : Anti-CEACAM1 antibody [EPR4048] (ab108390) at 1/1000 dilution
Lane 1 : His-tagged human CEAM-1 fragment
Lane 2 : His-tagged human CEAM-3 fragment
Lane 3 : His-tagged human CEAM-5 fragment
Lane 4 : His-tagged human CEAM-6 fragment
Lane 5 : His-tagged human CEAM-8 fragment
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 180 kDa (possible cross reactivity), 25 kDa (possible cross reactivity)
Exposure time: 37 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
This antibody cross-reacts with CEAM-3 and CEAM-5.
This data was developed using the same antibody clone in a different buffer formulation (ab108390).
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Immunohistochemical analysis of paraffin-embedded sections (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag. (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag. (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag. (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag. (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag. (F) HEK-293T cells transfected with empty vector containing a his tag.
This was labelling CEACAM1 with ab108390 at 1/2000 dilution. The section was incubated with ab108390 for 30 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counter stained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Positive staining on (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag and (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag. No staining on (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag and (F) HEK-293T cells transfected with empty vector containing a his tag. The results indicated cross reaction with CEACAM1, CEACAM3 and CEACAM5.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation (ab108390).
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All lanes : Anti-CEACAM1 antibody [EPR4048] (ab108390) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab108390).
Lanes 1 - 2: Merged signal (red and green). Green - ab108390 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108390 was shown to react with KDM5C / Jarid1C / SMCX in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108390 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab108390, at 1/100 dilution, staining CEACAM1 in Human kidney tissue by immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108390).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108390, at 1/100 dilution, staining CEACAM1 in Human liver tissue by immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108390).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab179460 has not yet been referenced specifically in any publications.