Product nameAnti-CEACAM1 antibody [EPR4049]
See all CEACAM1 primary antibodies
DescriptionRabbit monoclonal [EPR4049] to CEACAM1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human CEACAM1. The exact sequence is proprietary.
Database link: P13688
- WB: Human fetal liver, A549, SW480, and HepG2 cell lysates; IHC-P: Human kidney clear cell carcinoma tissue. IP: SW480 cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab108397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 58 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
- Information by UniProt
FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
All lanes : Anti-CEACAM1 antibody [EPR4049] (ab108397) at 1/10000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?
The smear and multiple bands are consistent with what have been described in the literature PMID: 17071610, 26448580 and 30050594, due to a variety of splice variants and/or differential glycosylation
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human clear cell carcinoma of kidney tissue sections labeling CEACAM1 with Purified ab108397 at 1:80 dilution (3.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
ab108397 (purified) at 1:20 dilution (1.5µg) immunoprecipitating CEACAM1 in SW480 whole cell lysate.
Lane 1 (input): SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab108397 & SW480 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108397 in SW480 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Unpurified ab108397 at 1/50 dilution staining CEACAM1 in Human kidney tissue by Immunohistochemistry, Paraffin-embedded tissue.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.