Key features and details
- Mouse monoclonal [GM8G5] to CEACAM1
- Suitable for: Flow Cyt, ELISA, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CEACAM1 antibody [GM8G5]
See all CEACAM1 primary antibodies
DescriptionMouse monoclonal [GM8G5] to CEACAM1
Specificityab26279 recognises CEACAM1 transiently expressed on the cell surface of transfected BOSC cells as well as the naturally expressed protein. As the A2 domain is unique to CEACAM1, ab26279 can be used to distinguish CEACAM1 from all other CEACAM.
Tested applicationsSuitable for: Flow Cyt, ELISA, IHC-Frmore details
Species reactivityReacts with: Human
Human CEACAM1-A2 domain cDNA (see relevance text).
ab26279 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human CEACAM1-A2 domain.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Concentration information loading...
PurityProtein G purified
Primary antibody notesab26279 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human CEACAM1-A2 domain.
Our Abpromise guarantee covers the use of ab26279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
- Information by UniProt
FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
Immunohistological staining of colon tissue with ab26279. Cryosections of colon tissue were stained with 10 ug/ml ab26279 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representative sections of analysed tissues. Detection of ab26279 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate. Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin.
ab26279 has been referenced in 2 publications.
- Thomas SN et al. Carcinoembryonic Antigen and CD44 Variant Isoforms Cooperate to Mediate Colon Carcinoma Cell Adhesion to E- and L-selectin in Shear Flow. J Biol Chem 283:15647-55 (2008). Flow Cyt ; Human . PubMed: 18375392
- Beaslas O et al. TRANSCRIPTOME RESPONSE OF ENTEROCYTES TO DIETARY LIPIDS: IMPACT ON CELL ARCHITECTURE, SIGNALING AND METABOLISM GENES. Am J Physiol Gastrointest Liver Physiol : (2008). PubMed: 18755805