Anti-CEACAM1 antibody - N-terminal (ab176018)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CEACAM1 antibody - N-terminal
See all CEACAM1 primary antibodies -
DescriptionRabbit polyclonal to CEACAM1 - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human CEACAM1 aa 56-86 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P13688 -
Positive control
- Human hepatocarcinoma tissue; HepG2 cell lysate; HepG2 cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityAmmonium Sulphate Precipitation
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab176018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 57 kDa. | |
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
- Information by UniProt
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Database links
- Entrez Gene: 634 Human
- Omim: 109770 Human
- SwissProt: P13688 Human
- Unigene: 512682 Human
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FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
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Alternative names
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
see all
Images
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Western blot - Anti-CEACAM1 antibody - N-terminal (ab176018)Anti-CEACAM1 antibody - N-terminal (ab176018) at 1/1000 dilution + HepG2 cell lysate at 35 µg
Predicted band size: 57 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody - N-terminal (ab176018)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue labeling CEACAM1 with ab176018 at 1/10 dilution, followed by peroxidase conjugation to the secondary antibody and DAB staining.
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Flow Cytometry - Anti-CEACAM1 antibody - N-terminal (ab176018)Flow cytometry analysis of HepG2 cells labeling CEACAM1 with ab176018 at 1/50 (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated Goat anti Rabbit secondary antibodies were used for the analysis.
Datasheets and documents
References
ab176018 has not yet been referenced specifically in any publications.
