Overview

  • Product name
    Anti-CEACAM1 antibody - N-terminal
    See all CEACAM1 primary antibodies
  • Description
    Rabbit polyclonal to CEACAM1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CEACAM1 aa 56-86 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P13688

  • Positive control
    • Human hepatocarcinoma tissue; HepG2 cell lysate; HepG2 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
  • Information by UniProt
  • Database links
  • Form
    There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
  • Alternative names
    • Antigen CD66 antibody
    • BGP 1 antibody
    • BGP antibody
    • BGP-1 antibody
    • BGPI antibody
    • Biliary glycoprotein 1 antibody
    • Biliary glycoprotein adhesion molecule antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 1 antibody
    • CD66a antibody
    • CD66a antigen antibody
    • CEACAM1 antibody
    • CEAM1_HUMAN antibody
    • meconium antigen 100 antibody
    see all

Images

  • Anti-CEACAM1 antibody - N-terminal (ab176018) at 1/1000 dilution + HepG2 cell lysate at 35 µg

    Predicted band size: 57 kDa

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue labeling CEACAM1 with ab176018 at 1/10 dilution, followed by peroxidase conjugation to the secondary antibody and DAB staining.

  • Flow cytometry analysis of HepG2 cells labeling CEACAM1 with ab176018 at 1/50 (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated Goat anti Rabbit secondary antibodies were used for the analysis.

References

ab176018 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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