Key features and details
- Rat monoclonal [YTH71.3] to CEACAM1
- Suitable for: IHC-P, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-CEACAM1 antibody [YTH71.3]
See all CEACAM1 primary antibodies
DescriptionRat monoclonal [YTH71.3] to CEACAM1
SpecificityRecognizes Human CEACAM1. CEACAM1 is expressed by Human neutrophils and cells in some cases of colon carcinoma. Reacts with transfectants containing BGPa, NCA and CGM1a which are CEA subfamily members.
Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey
ab104450 was raised against Human lymphocytes.
- Human Colon, Human Liver
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab104450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
- Information by UniProt
FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
Human Colon (formalin-fixed, paraffin-embedded) stained with ab104450 at 10 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Liver (formalin-fixed, paraffin-embedded) stained with ab104450 at 10 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab104450 has not yet been referenced specifically in any publications.