Overview

  • Product name

    Anti-CEACAM1 antibody [YTH71.3]
    See all CEACAM1 primary antibodies
  • Description

    Rat monoclonal [YTH71.3] to CEACAM1
  • Host species

    Rat
  • Specificity

    Recognizes Human CEACAM1. CEACAM1 is expressed by Human neutrophils and cells in some cases of colon carcinoma. Reacts with transfectants containing BGPa, NCA and CGM1a which are CEA subfamily members.
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    ab104450 was raised against Human lymphocytes.

  • Positive control

    • Human Colon, Human Liver

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    YTH71.3
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
  • Information by UniProt
  • Database links

  • Form

    There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
  • Alternative names

    • Antigen CD66 antibody
    • BGP 1 antibody
    • BGP antibody
    • BGP-1 antibody
    • BGPI antibody
    • Biliary glycoprotein 1 antibody
    • Biliary glycoprotein adhesion molecule antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 1 antibody
    • CD66a antibody
    • CD66a antigen antibody
    • CEACAM1 antibody
    • CEAM1_HUMAN antibody
    • meconium antigen 100 antibody
    see all

Images

  • Human Colon (formalin-fixed, paraffin-embedded) stained with ab104450 at 10 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Liver (formalin-fixed, paraffin-embedded) stained with ab104450 at 10 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab104450 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Serum (mouse serum (with pancreatic tumor development))
Loading amount
50 µg
Specification
mouse serum (with pancreatic tumor development)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 24 2012

Answer

Thank you for contacting us.

Neither antibody has been tested for reactivity with rabbit CEACAM1, and the epitopes each antibody recognizes have not been mapped, so it is difficult to predict cross-reactivity with rabbit. If I needed to choose one, I would choose ab49510, given the quality of the IHC image and the references. However, rabbit reactivity is not covered by our guarantee.

Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your inquiry. I was liaising with the laboratories to find out more about our pan-CEACAM- and CEACAM1-antibodies. Unfortunately, it turned out that we do not have any suitable antibody available: The immunogens of our CEACAM1 antibodies share a sequence homology with dog of 67% at the best. Thus, we would not predict a cross-reactivity for one of these antibodies. The same applies for our pan-CEACAM antibodies: The human CEACAM proteins used as immunogens reveal sequence similarities with the dog ortholog that are below 70%, suggesting that a cross-reactivity of the antibody is rather unlikely. If you have not done yet, I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I am sorry that I could not be more helpful on this occasion and hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

Read More

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