Overview

  • Product name
    Anti-CEACAM16 antibody [SU-9D5]
    See all CEACAM16 primary antibodies
  • Description
    Mouse monoclonal [SU-9D5] to CEACAM16
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, Competitive ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Genetic immunisation with cDNA encoding Human CEACAM16.

  • Positive control
    • BOSC23 cells transiently transfected with CEACAM16 expression vector; cochlea tissue from transgenic mice.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab167477 was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-PAGE.
  • Clonality
    Monoclonal
  • Clone number
    SU-9D5
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml.
Flow Cyt Use 1.2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Competitive ELISA 1/200 - 1/400.

Target

  • Relevance
    CEACAM16 is a single-pass type I membrane protein.It belongs to the immunoglobulin superfamily, CEA family. It contains 2 Ig-like C2-type (immunoglobulin-like) domains. The exact function of CEACAM16 remains unknown.
  • Cellular localization
    Membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • Carcinoembryonic antigen like 2 antibody
    • Carcinoembryonic antigen like 2 protein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 16 antibody
    • CEAL2 antibody
    • DFNA4B antibody
    see all

Images

  • Flow cytometric analysis of BOSC23 cells transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). ab167477 was used at 1.2 µg/106 cells and detection was with a PE- conjugated secondary antibody.
  • Immunohistochemical analysis of paraffin-embedded adult transgenic mouse cochlea tissue with a Human CEACAM16 gene, labeling CEACAM16 with ab167477 at 4 µg/ml. Binding of biotinylated antibody was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole. OH: Outer hair cells, TM: Tectorial membrane, RM: Reissner’s membrane.
  • BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, ab167477 was tested on all CEACAM transfectants at 1.2 µg/106 cells. A positive signal was obtained only with CEACAM16 transfected cells (red curve).

References

ab167477 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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