Overview

  • Product name
    Anti-CEACAM20 antibody [HT-12D8]
  • Description
    Mouse monoclonal [HT-12D8] to CEACAM20
  • Host species
    Mouse
  • Specificity
    ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, Competitive ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    cDNA encoding human CEACAM20

  • Positive control
    • CHO cells. FACS: BOSC23 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab59626 was purified by protein G affinity chromatography from cell culture supernatants.
  • Clonality
    Monoclonal
  • Clone number
    HT-12D8
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1.2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use 1µg for 106 cells.
Competitive ELISA 1/200 - 1/400.

Target

  • Relevance
    CEA-related cell adhesion molecule 20 (CEACAM20) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. The CEACAM20 protein contains a single N domain followed by 4 immunoglobulin-like A (A1, A2) and B (B1, B2) domains. Expression of CEACAM20 can be found in tissues of prostate, testis, duodenum and small intestine with highest expression in prostate. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. High expression of CEACAM20 in tissue of prostate carcinoma and in prostate carcinoma cell lines suggest that CEACAM20 can be used as a tumor marker.
  • Cellular localization
    Membrane; Single-pass type I membrane protein
  • Database links
    • Alternative names
      • carcinoembryonic antigen related cell adhesion molecule 20 antibody
      • UNQ9366 antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
      Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
      Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
    • Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
      Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
      BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).

    References

    ab59626 has not yet been referenced specifically in any publications.

    Publishing research using ab59626? Please let us know so that we can cite the reference in this datasheet.

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