Key features and details
- Mouse monoclonal [HT-12D8] to CEACAM20
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CEACAM20 antibody [HT-12D8]
DescriptionMouse monoclonal [HT-12D8] to CEACAM20
Specificityab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21.
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Other Immunogen Type corresponding to Human CEACAM20. cDNA encoding human CEACAM20
- CHO cells. FACS: BOSC23 cells
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein G purified
Purification notesab59626 was purified by protein G affinity chromatography from cell culture supernatants.
Our Abpromise guarantee covers the use of ab59626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use 1µg for 106 cells.|
RelevanceCEA-related cell adhesion molecule 20 (CEACAM20) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. The CEACAM20 protein contains a single N domain followed by 4 immunoglobulin-like A (A1, A2) and B (B1, B2) domains. Expression of CEACAM20 can be found in tissues of prostate, testis, duodenum and small intestine with highest expression in prostate. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. High expression of CEACAM20 in tissue of prostate carcinoma and in prostate carcinoma cell lines suggest that CEACAM20 can be used as a tumor marker.
Cellular localizationMembrane; Single-pass type I membrane protein
- carcinoembryonic antigen related cell adhesion molecule 20 antibody
- UNQ9366 antibody
Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).
ab59626 has not yet been referenced specifically in any publications.