Anti-CEACAM20 antibody [HT-12D8] (ab59626)
![Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)](https://www.abcam.com/ps/products/59/ab59626/Images/ab59626-73583-anti-ceacam20-antibody-ht-12d8-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)")
Key features and details
- Mouse monoclonal [HT-12D8] to CEACAM20
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CEACAM20 antibody [HT-12D8] -
Description
Mouse monoclonal [HT-12D8] to CEACAM20 -
Host species
Mouse -
Specificity
ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21. -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human CEACAM20. cDNA encoding human CEACAM20
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Positive control
- CHO cells. FACS: BOSC23 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab59626 was purified by protein G affinity chromatography from cell culture supernatants. -
Clonality
Monoclonal -
Clone number
HT-12D8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab59626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1.2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use 1µg for 106 cells. |
Target
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Relevance
CEA-related cell adhesion molecule 20 (CEACAM20) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. The CEACAM20 protein contains a single N domain followed by 4 immunoglobulin-like A (A1, A2) and B (B1, B2) domains. Expression of CEACAM20 can be found in tissues of prostate, testis, duodenum and small intestine with highest expression in prostate. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. High expression of CEACAM20 in tissue of prostate carcinoma and in prostate carcinoma cell lines suggest that CEACAM20 can be used as a tumor marker. -
Cellular localization
Membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 125931 Human
- SwissProt: Q6UY09 Human
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Alternative names
- carcinoembryonic antigen related cell adhesion molecule 20 antibody
- UNQ9366 antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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![Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)](https://www.abcam.com/ps/products/59/ab59626/Images/ab59626-73582-anti-ceacam20-antibody-ht-12d8-flow-cytometry.jpg)
Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).
Protocols
Datasheets and documents
References (0)
ab59626 has not yet been referenced specifically in any publications.
