Key features and details
- Rabbit polyclonal to CEACAM3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CEACAM3 antibody
See all CEACAM3 primary antibodies
DescriptionRabbit polyclonal to CEACAM3
Specificityab196606 detects endogenous level of total CEACAM3 protein
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human CEACAM3 (N terminal). The exact sequence is proprietary.
Database link: P40198
- A549 whole cell lysate (ab7910) can be used as a positive control in WB. Paraffin-embedded human epididymis, Human lung and Human testis tissue. A459, HT29, Mous eliver, Mouse lung and Mouse testis lysates.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 27 kDa.|
FunctionMajor granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.
Tissue specificityCGM1a, the predominant CGM1 transcript, is granulocyte-specific. Not detected out of the granulocytic lineage, such as monocytes, lymphocytes, spleen, testis, colon, brain, liver, pancreas, thymus, ovary, placenta, skeletal muscle, prostate, small intestine, heart, lung and kidney.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe cytosolic domain is involved in S100A9 interaction.
modificationsTyrosine-phosphorylated in response to microbial binding. Tyr-230 and Tyr-241 are both required for phosphorylation to be detected.
- Information by UniProt
- carcinoembryonic antibody
- Carcinoembryonic antigen CGM1 antibody
- Carcinoembryonic antigen gene family member 1 antibody
All lanes : Anti-CEACAM3 antibody (ab196606) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : HT29 cell lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse lung lysate
Lane 5 : Mouse testis lysate
Predicted band size: 27 kDa
Immunohistochemical analysis of paraffin-embedded human epididymis labeling CEACAM3 using ab196606 at 1/100 dilution
Immunohistochemical analysis of paraffin-embedded human lung labeling CEACAM3 using ab196606 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human testis labeling CEACAM3 using ab196606 at 1/100 dilution.
ab196606 has not yet been referenced specifically in any publications.