Recombinant
RabMAb

Recombinant Anti-CEACAM6 antibody [EPR4403] (ab134074)

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Overview

  • Product name

    Anti-CEACAM6 antibody [EPR4403]
    See all CEACAM6 primary antibodies

  • Description

    Rabbit monoclonal [EPR4403] to CEACAM6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CEACAM6 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human placenta, JAR, HT29, and MCF7 cell lysates; Human colon tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4403

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 45-90 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. CEA family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
        • Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
        • Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
        • CD 66c antibody
        • CD66c antibody
        • CD66c antigen antibody
        • CEA LIKE PROTEIN antibody
        • CEACAM 6 antibody
        • CEACAM6 antibody
        • CEAL antibody
        • CEAM6_HUMAN antibody
        • MGC93832 antibody
        • NCA antibody
        • Non specific cross reacting antigen antibody
        • Non-specific crossreacting antigen antibody
        • Normal cross reacting antigen antibody
        • Normal cross-reacting antigen antibody
        see all

      Images

      • Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)
        Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)
        All lanes : Anti-CEACAM6 antibody [EPR4403] (ab134074) at 1/10000 dilution

        Lane 1 : Human placenta cell lysate
        Lane 2 : JAR cell lysate
        Lane 3 : HT29 cell lysate
        Lane 4 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

        Predicted band size: 37 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (ab134074)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (ab134074)

        Immunohistochemical analysis of paraffin embedded human colon tissue labelling CEACAM6 with ab134074 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Rodrigues C  et al. A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells. Exp Cell Res 378:206-216 (2019). Read more (PubMed: 30772380) »
      • Jin C  et al. T cell immunity induced by a bivalent Salmonella-based CEACAM6 and 4-1BBL vaccines in a rat colorectal cancer model. Oncol Lett 13:3753-3759 (2017). Read more (PubMed: 28521477) »
      See all 3 Publications for this product

      Publishing research using ab134074? Please let us know so that we can cite the reference in this datasheet.

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