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Tags & Cell Markers Cell Type Markers Tumor Associated
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RecombinantRabMAb

Recombinant Anti-CEACAM6 antibody [EPR4403] (ab134074)

  • Datasheet
  • SDS
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Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (ab134074)
  • Anti-CEACAM6 antibody [EPR4403] (ab134074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4403] to CEACAM6
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Protein
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Recombinant human CEACAM6 protein (ab167705)

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Overview

  • Product name

    Anti-CEACAM6 antibody [EPR4403]
    See all CEACAM6 primary antibodies
  • Description

    Rabbit monoclonal [EPR4403] to CEACAM6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CEACAM6 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human placenta, JAR, HT29, and MCF7 cell lysates; Human colon tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4403
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CEACAM6 antibody [EPR4403] (ab205464)
    • Alexa Fluor® 647 Anti-CEACAM6 antibody [EPR4403] (ab205465)
    • Anti-CEACAM6 antibody [EPR4403] - BSA and Azide free (ab235139)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
  • Recombinant Protein

    • Recombinant human CEACAM6 protein (ab167705)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 45-90 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 45-90 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. CEA family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P40199 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4680 Human
      • Entrez Gene: 100125369 Rat
      • Omim: 163980 Human
      • SwissProt: P40199 Human
      • Unigene: 466814 Human
      • Unigene: 122781 Rat
      • Alternative names

        • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
        • Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
        • Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
        • CD 66c antibody
        • CD66c antibody
        • CD66c antigen antibody
        • CEA LIKE PROTEIN antibody
        • CEACAM 6 antibody
        • CEACAM6 antibody
        • CEAL antibody
        • CEAM6_HUMAN antibody
        • MGC93832 antibody
        • NCA antibody
        • Non specific cross reacting antigen antibody
        • Non-specific crossreacting antigen antibody
        • Normal cross reacting antigen antibody
        • Normal cross-reacting antigen antibody
        see all

      Images

      • Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)
        Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)
        All lanes : Anti-CEACAM6 antibody [EPR4403] (ab134074) at 1/10000 dilution

        Lane 1 : Human placenta cell lysate
        Lane 2 : JAR cell lysate
        Lane 3 : HT29 cell lysate
        Lane 4 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

        Predicted band size: 37 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (ab134074)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (ab134074)

        Immunohistochemical analysis of paraffin embedded human colon tissue labelling CEACAM6 with ab134074 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-CEACAM6 antibody [EPR4403] (ab134074)
        Anti-CEACAM6 antibody [EPR4403] (ab134074)

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab134074? Please let us know so that we can cite the reference in this datasheet.

      ab134074 has been referenced in 3 publications.

      • Rodrigues C  et al. A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells. Exp Cell Res 378:206-216 (2019). PubMed: 30772380
      • Jin C  et al. T cell immunity induced by a bivalent Salmonella-based CEACAM6 and 4-1BBL vaccines in a rat colorectal cancer model. Oncol Lett 13:3753-3759 (2017). PubMed: 28521477
      • Liu Y  et al. FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer. Oncol Lett 11:3693-3701 (2016). IHC . PubMed: 27284373

      Customer reviews and Q&As

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      For licensing inquiries, please contact partnerships@abcam.com

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