Overview

  • Product name

    Anti-CEACAM6 antibody [EPR4403]
    See all CEACAM6 primary antibodies
  • Description

    Rabbit monoclonal [EPR4403] to CEACAM6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CEACAM6 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human placenta, JAR, HT29, and MCF7 cell lysates; Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4403
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 45-90 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. CEA family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
      • Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
      • Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
      • CD 66c antibody
      • CD66c antibody
      • CD66c antigen antibody
      • CEA LIKE PROTEIN antibody
      • CEACAM 6 antibody
      • CEACAM6 antibody
      • CEAL antibody
      • CEAM6_HUMAN antibody
      • MGC93832 antibody
      • NCA antibody
      • Non specific cross reacting antigen antibody
      • Non-specific crossreacting antigen antibody
      • Normal cross reacting antigen antibody
      • Normal cross-reacting antigen antibody
      see all

    Images

    • All lanes : Anti-CEACAM6 antibody [EPR4403] (ab134074) at 1/10000 dilution

      Lane 1 : Human placenta cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HT29 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Immunohistochemical analysis of paraffin embedded human colon tissue labelling CEACAM6 with ab134074 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Rodrigues C  et al. A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells. Exp Cell Res 378:206-216 (2019). Read more (PubMed: 30772380) »
    • Jin C  et al. T cell immunity induced by a bivalent Salmonella-based CEACAM6 and 4-1BBL vaccines in a rat colorectal cancer model. Oncol Lett 13:3753-3759 (2017). Read more (PubMed: 28521477) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134074.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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