Recombinant Anti-CEACAM6 antibody [EPR4403] (ab134074)
![Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)](https://www.abcam.com/ps/products/134/ab134074/Images/ab134074-148210-anti-ceacam6-antibody-epr4403-western-blot.jpg "Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4403] to CEACAM6
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CEACAM6 antibody [EPR4403]
See all CEACAM6 primary antibodies -
Description
Rabbit monoclonal [EPR4403] to CEACAM6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human CEACAM6 aa 200-300. The exact sequence is proprietary.
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Positive control
- Human placenta, JAR, HT29, and MCF7 cell lysates; Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4403 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 45-90 kDa (predicted molecular weight: 37 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 45-90 kDa (predicted molecular weight: 37 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4680 Human
- Entrez Gene: 100125369 Rat
- Omim: 163980 Human
- SwissProt: P40199 Human
- Unigene: 466814 Human
- Unigene: 122781 Rat
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Alternative names
- Carcinoembryonic antigen related cell adhesion molecule 6 antibody
- Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
- Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
see all
Images
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Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074)All lanes : Anti-CEACAM6 antibody [EPR4403] (ab134074) at 1/10000 dilution
Lane 1 : Human placenta cell lysate
Lane 2 : JAR cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (ab134074)](https://www.abcam.com/ps/products/134/ab134074/Images/ab134074-148209-anti-ceacam6-antibody-epr4403-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (ab134074)Immunohistochemical analysis of paraffin embedded human colon tissue labelling CEACAM6 with ab134074 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-CEACAM6 antibody [EPR4403] (ab134074)](https://www.abcam.com/ps/products/134/ab134074/Images/ab134074-3-benefits-of-recombinant-antibodies.png)
Anti-CEACAM6 antibody [EPR4403] (ab134074)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134074 has been referenced in 3 publications.
- Rodrigues C et al. A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells. Exp Cell Res 378:206-216 (2019). PubMed: 30772380
- Jin C et al. T cell immunity induced by a bivalent Salmonella-based CEACAM6 and 4-1BBL vaccines in a rat colorectal cancer model. Oncol Lett 13:3753-3759 (2017). PubMed: 28521477
- Liu Y et al. FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer. Oncol Lett 11:3693-3701 (2016). IHC . PubMed: 27284373