Anti-CEACAM7 antibody [BAC2] (ab26281)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-CEACAM7 antibody [BAC2]
See all CEACAM7 primary antibodies -
DescriptionMouse monoclonal [BAC2] to CEACAM7
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Host speciesMouse
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Specificityab26281 recognizes exclusively CEACAM7 transiently expressed on the cell surface of transfected BOSC23 cells. It can be used to distinguish CEACAM7 from all other CEACAM and probably all pregnancy-specific glycoprotein molecules, namely CEACAM1 (BGP/CD66a), CEACAM3 (CGM1/CD66d), CEACAM4 (CGM7), CEACAM6 (NCA/CD66c), CEACAM7 (CGM2), CEACAM8 (CGM6/CD66b) and PSG1 (CD66f) based on its reactivity pattern with stable and transiently transfected mammalian cells expressing individual CEA family members.
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Tested applicationsSuitable for: IHC-P, Flow Cyt, ELISA, IHC-Frmore details
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Species reactivityReacts with: Human
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Immunogen
ab26281 was generated by immunisation of BALB/c mice with a CEACAM7/Fc fusion protein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberBAC2
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab26281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Tissue specificityStrongly down-regulated in colonic adenocarcinomas.
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Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1087 Human
- SwissProt: Q14002 Human
- Unigene: 74466 Human
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Alternative names
- Carcinoembryonic antigen CGM 2 antibody
- Carcinoembryonic antigen CGM2 antibody
- Carcinoembryonic antigen gene family member 2 antibody
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Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM7 antibody [BAC2] (ab26281)](https://www.abcam.com/ps/datasheet/images/26/ab26281/CEACAM7-Primary-antibodies-ab26281-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM7 antibody [BAC2] (ab26281)Image from Zhou J et al., World J Surg Oncol. 2011 Dec 23;9:172. Fig 3.; doi:10.1186/1477-7819-9-172; 23 December 2011, World Journal of Surgical Oncology 2011, 9:172Immunohistochemical analysis of gastric carcinoma tissue, stainin CEACAM7 with ab26281.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Sections were blocked with 10% normal goat serum in PBS for 30 minutes followed by incubation overnight at 4°C with the primary antibody (1/100 in 1% BSA). Staining was detected using DAB.
References
This product has been referenced in:
- Kobayashi M et al. Carcinoembryonic antigen-related cell adhesion molecules as surrogate markers for EGFR inhibitor sensitivity in human lung adenocarcinoma. Br J Cancer 107:1745-53 (2012). Read more (PubMed: 23099808) »
- Zhou J et al. Dynamic expression of CEACAM7 in precursor lesions of gastric carcinoma and its prognostic value in combination with CEA. World J Surg Oncol 9:172 (2011). IHC-P ; Human . Read more (PubMed: 22195770) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"