Overview

  • Product name

  • Description

    Rabbit polyclonal to CEAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide within Human CEAP aa 21-50 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. NP_776170.2.
    Database link: Q6QNY1

  • Positive control

    • HepG2 cell lysate, NIH 3T3 cell lysate, Human cervix carcinoma tissue and HepG2 cells.
  • General notes

     This product was previously labelled as BLOC1S2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab174929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 16 kDa.

Target

  • Function

    May play a role in cell proliferation. The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking.
  • Tissue specificity

    Isoform 1 and isoform 2 are widely expressed. Expressed in various malignant tumor tissues (at protein level).
  • Sequence similarities

    Belongs to the BLOC1S2 family.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Localizes to the centrosomes in a microtubule-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • Biogenesis of lysosome-related organelles complex 1 subunit 2 antibody
    • BL1S2_HUMAN antibody
    • BLOC-1 subunit 2 antibody
    • Bloc1s2 antibody
    • BLOS2 antibody
    • Centrosome-associated protein antibody
    • RP11 316M21.4 antibody
    see all

Images

  • Anti-CEAP antibody (ab174929) at 1/1000 dilution + NIH 3T3 cell lysate at 15 µg

    Predicted band size: 16 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human cervix carcinoma tissue labeling CEAP using ab174929 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram) using ab174929 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-CEAP antibody (ab174929) at 1/1000 dilution + HepG2 cell lysate at 15 µg

    Predicted band size: 16 kDa

References

ab174929 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab174929.
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