Anti-CEAP antibody (ab174929)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CEAP antibody
See all CEAP primary antibodies -
Description
Rabbit polyclonal to CEAP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey
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Immunogen
Synthetic peptide within Human CEAP aa 21-50 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. NP_776170.2.
Database link: Q6QNY1 -
Positive control
- HepG2 cell lysate, NIH 3T3 cell lysate, Human cervix carcinoma tissue and HepG2 cells.
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General notes
This product was previously labelled as BLOC1S2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab174929 is purified through a protein A column, followed by peptide affinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab174929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 16 kDa. |
Target
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Function
May play a role in cell proliferation. The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. -
Tissue specificity
Isoform 1 and isoform 2 are widely expressed. Expressed in various malignant tumor tissues (at protein level). -
Sequence similarities
Belongs to the BLOC1S2 family. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Localizes to the centrosomes in a microtubule-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 282991 Human
- Entrez Gene: 73689 Mouse
- Omim: 609768 Human
- SwissProt: Q6QNY1 Human
- SwissProt: Q9CWG9 Mouse
- Unigene: 34906 Human
- Unigene: 124685 Mouse
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Alternative names
- Biogenesis of lysosome-related organelles complex 1 subunit 2 antibody
- BL1S2_HUMAN antibody
- BLOC-1 subunit 2 antibody
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Images
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Western blot - Anti-CEAP antibody (ab174929)Anti-CEAP antibody (ab174929) at 1/1000 dilution + NIH 3T3 cell lysate at 15 µg
Predicted band size: 16 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEAP antibody (ab174929)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human cervix carcinoma tissue labeling CEAP using ab174929 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow Cytometry - Anti-CEAP antibody (ab174929)Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram) using ab174929 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot - Anti-CEAP antibody (ab174929)Anti-CEAP antibody (ab174929) at 1/1000 dilution + HepG2 cell lysate at 15 µg
Predicted band size: 16 kDa
Datasheets and documents
References
ab174929 has not yet been referenced specifically in any publications.
