Recombinant Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CEBP Alpha/CEBPA antibody [EP708Y]
See all CEBP Alpha/CEBPA primary antibodies -
Description
Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA -
Host species
Rabbit -
Specificity
This antibody recognizes isoform1 and isoform 4.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CEBP Alpha/CEBPA aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- U937+LPS cell lysate, HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP708Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 43 kDa).
|
ICC/IF |
1/250 - 1/500.
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Notes |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 43 kDa). |
ICC/IF
1/250 - 1/500. |
Target
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Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1050 Human
- Omim: 116897 Human
- SwissProt: P49715 Human
- Unigene: 76171 Human
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Alternative names
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
see all
Images
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Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg
Predicted band size: 43 kDa
Observed band size: 30,43 kDa why is the actual band size different from the predicted?We are unsure as to the identity of the 30kda band.
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ab40761 (1/250) staining HeLa cells using immunofluorescence.
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Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells
Secondary
HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocked with 5% Milk for 1 hour at 24°C.
We are unsure as to the identity of the 30kda band.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab40761 has been referenced in 18 publications.
- Zhang LM et al. CCAAT enhancer binding protein α suppresses proliferation, metastasis, and epithelial-mesenchymal transition of ovarian cancer cells via suppressing the Wnt/β-catenin signaling. Neoplasma 68:602-612 (2021). PubMed: 33780265
- Ni T et al. Dihydrolycorine Attenuates Cardiac Fibrosis and Dysfunction by Downregulating Runx1 following Myocardial Infarction. Oxid Med Cell Longev 2021:8528239 (2021). PubMed: 34725565
- Pope BD et al. Fattening chips: hypertrophy, feeding, and fasting of human white adipocytes in vitro. Lab Chip 20:4152-4165 (2020). PubMed: 33034335
- Zhang W et al. A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-ß expression. Nutr Metab (Lond) 16:88 (2019). PubMed: 31889968
- Ptasinska A et al. RUNX1-ETO Depletion in t(8;21) AML Leads to C/EBPa- and AP-1-Mediated Alterations in Enhancer-Promoter Interaction. Cell Rep 28:3022-3031.e7 (2019). PubMed: 31533028