Overview

  • Product name

    Anti-CEBP Alpha/CEBPA antibody
    See all CEBP Alpha/CEBPA primary antibodies
  • Description

    Rabbit polyclonal to CEBP Alpha/CEBPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human CEBP Alpha/CEBPA aa 250-350 conjugated to keyhole limpet haemocyanin.
    Database link: P49715

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Kidney, Mouse Kidney, Human Pancreas, Human Liver. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.
  • General notes

     This product was previously labelled as CEBP Alpha

     

Properties

Applications

Our Abpromise guarantee covers the use of ab140479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
  • Sequence similarities

    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch 5 antibody
    • C/EBP alpha antibody
    • C/ebpalpha antibody
    • CAP4 antibody
    • Caspase 8 precursor antibody
    • CBF-A antibody
    • CCAAT Enhancer Binding Protein alpha antibody
    • CCAAT/enhancer binding protein (C/EBP), alpha antibody
    • CCAAT/enhancer-binding protein alpha antibody
    • CEBP antibody
    • CEBP A antibody
    • CEBP alpha antibody
    • Cebpa antibody
    • CEBPA_HUMAN antibody
    • FADD homologous ICE/CED 3 like protease antibody
    • FADD like ICE antibody
    • FLICE antibody
    • ICE like apoptotic protease 5 antibody
    • ICE8 antibody
    • MACH antibody
    • MCH5 antibody
    • MORT1 associated CED 3 homolog antibody
    see all

Images

  • IHC image of CEBP Alpha/CEBPA staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140479, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-CEBP Alpha/CEBPA antibody (ab140479) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Kidney (Mouse) Tissue Lysate (ab27254)
    Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 40 kDa (possible non-specific binding), 53 kDa (possible non-specific binding)


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140479 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab140479 has not yet been referenced specifically in any publications.

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