Recombinant
RabMAb

Recombinant Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

Overview

  • Product name

    Anti-CEBP Alpha/CEBPA antibody [EP708Y]
    See all CEBP Alpha/CEBPA primary antibodies
  • Description

    Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes isoform1 and isoform 4.

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CEBP Alpha/CEBPA aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • U937+LPS cell lysate, HeLa cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as CEBP Alpha

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC or IP.
  • Target

    • Function

      C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
    • Sequence similarities

      Belongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch 5 antibody
      • C/EBP alpha antibody
      • C/ebpalpha antibody
      • CAP4 antibody
      • Caspase 8 precursor antibody
      • CBF-A antibody
      • CCAAT Enhancer Binding Protein alpha antibody
      • CCAAT/enhancer binding protein (C/EBP), alpha antibody
      • CCAAT/enhancer-binding protein alpha antibody
      • CEBP antibody
      • CEBP A antibody
      • CEBP alpha antibody
      • Cebpa antibody
      • CEBPA_HUMAN antibody
      • FADD homologous ICE/CED 3 like protease antibody
      • FADD like ICE antibody
      • FLICE antibody
      • ICE like apoptotic protease 5 antibody
      • ICE8 antibody
      • MACH antibody
      • MCH5 antibody
      • MORT1 associated CED 3 homolog antibody
      see all

    Images

    • Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg

      Predicted band size: 43 kDa
      Observed band size: 30,43 kDa
      why is the actual band size different from the predicted?



      We are unsure as to the identity of the 30kda band.

    • ab40761 (1/250) staining HeLa cells using immunofluorescence.
    • Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells

      Secondary
      HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocked with 5% Milk for 1 hour at 24°C.

      We are unsure as to the identity of the 30kda band.

      See Abreview

    References

    This product has been referenced in:

    • Scully KM  et al. E47 Governs the MYC-CDKN1B/p27KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16INK4A and Wild-Type p53. Cell Mol Gastroenterol Hepatol 6:181-198 (2018). Read more (PubMed: 30003124) »
    • Smatlikova P  et al. Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Pig Transgenic Model Expressing Human Mutant Huntingtin. J Huntingtons Dis N/A:N/A (2018). Read more (PubMed: 30584151) »
    See all 11 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100000 cells
    Specification
    MCF7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted May 11 2012

    Answer

    Thank you for contacting Abcam.

    We do not currently have an image of how ab40761 works in western blot on HeLa cells, all of the western blot data we have for this antibody is on our website. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples.

    If there is anything else I can help you with, please let me know.

    Read More
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT1080 Cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    200000 cells
    Specification
    HT1080 Cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jan 04 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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