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    cebp-alphacebpa-antibody-ep709y-ab40764.pdf

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RecombinantRabMAb

Recombinant Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (28)

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Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
  • Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP709Y] to CEBP Alpha/CEBPA
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human CEBP Alpha/CEBPA protein (ab51301)

View more associated products

Overview

  • Product name

    Anti-CEBP Alpha/CEBPA antibody [EP709Y]
    See all CEBP Alpha/CEBPA primary antibodies
  • Description

    Rabbit monoclonal [EP709Y] to CEBP Alpha/CEBPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human CEBP Alpha/CEBPA aa 300-400 (C terminal).

  • Positive control

    • U937 cell lysate
  • General notes

    This product was previously labelled as CEBP Alpha

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP709Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Other
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-CEBP Alpha/CEBPA antibody [EP709Y] - BSA and Azide free (ab219726)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CEBP Alpha/CEBPA protein (ab51301)

Applications

Our Abpromise guarantee covers the use of ab40764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
    • Sequence similarities

      Belongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P49715 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1050 Human
      • Entrez Gene: 12606 Mouse
      • Entrez Gene: 24252 Rat
      • Omim: 116897 Human
      • SwissProt: P49715 Human
      • SwissProt: P53566 Mouse
      • SwissProt: P05554 Rat
      • Unigene: 76171 Human
      • Unigene: 349667 Mouse
      • Unigene: 204833 Rat
      see all
    • Alternative names

      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch 5 antibody
      • C/EBP alpha antibody
      • C/ebpalpha antibody
      • CAP4 antibody
      • Caspase 8 precursor antibody
      • CBF-A antibody
      • CCAAT Enhancer Binding Protein alpha antibody
      • CCAAT/enhancer binding protein (C/EBP), alpha antibody
      • CCAAT/enhancer-binding protein alpha antibody
      • CEBP antibody
      • CEBP A antibody
      • CEBP alpha antibody
      • Cebpa antibody
      • CEBPA_HUMAN antibody
      • FADD homologous ICE/CED 3 like protease antibody
      • FADD like ICE antibody
      • FLICE antibody
      • ICE like apoptotic protease 5 antibody
      • ICE8 antibody
      • MACH antibody
      • MCH5 antibody
      • MORT1 associated CED 3 homolog antibody
      see all

    Images

    • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
      Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
      All lanes : Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764) at 1/1000 dilution (Purified)

      Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysates
      Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
      Lane 3 : Mouse liver lysates
      Lane 4 : Rat liver lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa



      This antibody detects high backgrounds

    • Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
      Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (28)

    Publishing research using ab40764? Please let us know so that we can cite the reference in this datasheet.

    ab40764 has been referenced in 28 publications.

    • Shi Y  et al. miR-196b-5p controls adipocyte differentiation and lipogenesis through regulating mTORC1 and TGF-ß signaling. FASEB J N/A:N/A (2020). PubMed: 32469097
    • Yuan H  et al. Hypomethylation of nerve growth factor (NGF) promotes binding of C/EBPa and contributes to inflammatory hyperalgesia in rats. J Neuroinflammation 17:34 (2020). PubMed: 31980031
    • Qin Y  et al. Alterations in promoter interaction landscape and transcriptional network underlying metabolic adaptation to diet. Nat Commun 11:962 (2020). PubMed: 32075973
    • Jiang T  et al. Isoimperatorin enhances 3T3-L1 preadipocyte differentiation by regulating PPAR? and C/EBPa through the Akt signaling pathway. Exp Ther Med 18:2160-2166 (2019). PubMed: 31452707
    • Guan F  et al. New Molecular Mechanism Underlying Myc-Mediated Cytochrome P450 2E1 Upregulation in Apoptosis and Energy Metabolism in the Myocardium. J Am Heart Assoc 8:e009871 (2019). PubMed: 30563421
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-CEBP Alpha/CEBPA antibody [EP709Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (fat)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    20 µg
    Specification
    fat
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Western blot abreview for Anti-CEBP Alpha/CEBPA antibody [EP709Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ESC (HUES7))
    Gel Running Conditions
    Non-reduced Denaturing (12% gel)
    Loading amount
    10 µg
    Specification
    ESC (HUES7)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 09 2016

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