Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
- Datasheet
- References (13)
- Protocols
Overview
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Product nameAnti-CEBP Alpha/CEBPA antibody [EP709Y]
See all CEBP Alpha/CEBPA primary antibodies -
DescriptionRabbit monoclonal [EP709Y] to CEBP Alpha/CEBPA
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Host speciesRabbit
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Tested applicationsSuitable for: EMSA, WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
corresponding to Human CEBP Alpha/CEBPA aa 300-400 (C terminal).
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Positive control
- U937 cell lysate
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP709Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab40764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| EMSA | Use at an assay dependent concentration. PubMed: 22184113 | |
| WB | 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionC/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
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Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1050 Human
- Entrez Gene: 12606 Mouse
- Entrez Gene: 24252 Rat
- Omim: 116897 Human
- SwissProt: P49715 Human
- SwissProt: P53566 Mouse
- SwissProt: P05554 Rat
- Unigene: 76171 Human
see all -
Alternative names
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
see all
Images
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![Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)](https://www.abcam.com/ps/products/40/ab40764/Images/ab40764.bmp)
Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764) at 1/20000 dilution + U937 cell lysate at 10 µg
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 12 seconds -
![Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)](https://www.abcam.com/ps/products/40/ab40764/Images/ab40764-273263-ab4076455606.jpg)
Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)This image is courtesy of an anonymous abreview.Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764) at 1/1000 dilution + Human ESC (HUES7) whole cell lysate at 10 µg with 5% milk for 30 minutes at RT.
Secondary
Monoclonal Donkey Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 43 kDa
References
This product has been referenced in:
- Sun Y et al. Arctigenin Inhibits Liver Cancer Tumorigenesis by Inhibiting Gankyrin Expression via C/EBPa and PPARa. Front Pharmacol 9:268 (2018). Read more (PubMed: 29636686) »
- Xiao H et al. Increased H3K27ac level of ACE mediates the intergenerational effect of low peak bone mass induced by prenatal dexamethasone exposure in male offspring rats. Cell Death Dis 9:638 (2018). Read more (PubMed: 29844424) »
