Anti-CEBP Beta antibody [18F8] (ab15049)
![Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)](https://www.abcam.com/ps/products/15/ab15049/Images/ab15049-22280-anti-cebp-beta-antibody-18f8-western-blot.jpg "Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)")
Key features and details
- Mouse monoclonal [18F8] to CEBP Beta
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-CEBP Beta antibody [18F8]
See all CEBP Beta primary antibodies -
Description
Mouse monoclonal [18F8] to CEBP Beta -
Host species
Mouse -
Specificity
Ab15049 only detects the LAP* isoform of CEBPG beta. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
Synthetic peptide corresponding to Mouse CEBP Beta aa 1-19 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:MHRLLAWDAACLPPPPAAF
(Peptide available asab39810) -
Positive control
- Transfected 293T cells, P388D1 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
18F8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810).
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810). |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. -
Tissue specificity
Expressed at low levels in the lung, kidney and spleen. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsSumoylated by polymeric chains of SUMO2 or SUMO3. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 12608 Mouse
- SwissProt: P28033 Mouse
- Unigene: 439656 Mouse
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Alternative names
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
see all
Images
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Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)
Western blot of the CEBP Beta using ab15049 on transfected CEBP beta.
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![Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)](https://www.abcam.com/ps/products/15/ab15049/Images/ab15049-22279-anti-cebp-beta-antibody-18f8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)Immunofluorescence staining of CEBP beta in P388D1 cells using ab15049.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab15049 has been referenced in 7 publications.
- Liu SP et al. Secukinumab attenuates reactive astrogliosis via IL-17RA/(C/EBPß)/SIRT1 pathway in a rat model of germinal matrix hemorrhage. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31020769
- Romanel A et al. Inherited determinants of early recurrent somatic mutations in prostate cancer. Nat Commun 8:48 (2017). PubMed: 28663546
- Jang MA et al. a-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS One 12:e0170699 (2017). PubMed: 28114367
- Liu Q et al. Cyclin D1 and C/EBPß LAP1 operate in a common pathway to promote mammary epithelial cell differentiation. Mol Cell Biol 34:3168-79 (2014). PubMed: 24912680
- Atwood AA et al. Negative Regulation of C/EBPbeta1 by Sumoylation in Breast Cancer Cells. PLoS One 6:e25205 (2011). WB ; Human . PubMed: 21980398
- Atwood AA & Sealy L Regulation of C/EBPbeta1 by Ras in mammary epithelial cells and the role of C/EBPbeta1 in oncogene-induced senescence. Oncogene 29:6004-15 (2010). WB ; Human . PubMed: 20818427
- Su WC et al. Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene. J Biol Chem 278:51150-8 (2003). PubMed: 14530280