Overview

  • Product name
    Anti-CEBP Beta antibody [18F8]
    See all CEBP Beta primary antibodies
  • Description
    Mouse monoclonal [18F8] to CEBP Beta
  • Host species
    Mouse
  • Specificity
    Ab15049 only detects the LAP* isoform of CEBPG beta.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse CEBP Beta aa 1-19 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MHRLLAWDAACLPPPPAAF


    (Peptide available as ab39810)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Transfected 293T cells, P388D1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • Tissue specificity
    Expressed at low levels in the lung, kidney and spleen.
  • Sequence similarities
    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Domain
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AGP/EBP antibody
    • C EBP beta antibody
    • C/EBP beta antibody
    • C/EBP related protein 2 antibody
    • CCAAT Enhancer Binding Protein beta antibody
    • CCAAT/enhancer-binding protein beta antibody
    • CEBPB antibody
    • CEBPB_HUMAN antibody
    • CRP2 antibody
    • IL 6DBP antibody
    • IL6DBP antibody
    • Interleukin 6 dependent binding protein antibody
    • LAP antibody
    • Liver activator protein antibody
    • Liver enriched transcriptional activator antibody
    • NF IL6 antibody
    • NFIL6 antibody
    • Nuclear factor NF IL6 antibody
    • Nuclear factor NF-IL6 antibody
    • SF B antibody
    • SFB antibody
    • Silencer factor B antibody
    • TCF-5 antibody
    • TCF5 antibody
    • Transcription factor 5 antibody
    see all

Images

  • Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)
    Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)

    Western blot of the CEBP Beta using ab15049 on transfected CEBP beta.

  • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)
    Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)
    Immunofluorescence staining of CEBP beta in P388D1 cells using ab15049.

References

This product has been referenced in:
  • Jang MA  et al. a-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS One 12:e0170699 (2017). Read more (PubMed: 28114367) »
  • Liu Q  et al. Cyclin D1 and C/EBPß LAP1 operate in a common pathway to promote mammary epithelial cell differentiation. Mol Cell Biol 34:3168-79 (2014). Read more (PubMed: 24912680) »

See all 5 Publications for this product

Publishing research using ab15049? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Inquiry: I have C/EBP beta antibody (ab15049). data sheet say that ab15049 only detect the LAP isoform of C/EBP beta. C/EBP beta has three isoforms like FL (full length), LAP and LIP. Sometimes, in western blot, FL and LAP can be hard to be distinguish...

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There are three isoforms of C/EBP Beta; LAP*(38kDa), LAP(35kDa) and LIP(20 kDa). Our information specifies that this antibody only detects LAP* or full length as you describe. The immunogen used to make this antibody covers amino acids 1-21 of the full...

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Unfortunately I have already ordered and paid for this. Do you have a CEBPG antibody, poly or monoclonal which I can order? Can I assume that I will will be reimbursed for my order?

I sincerely apologize for the error. I will ask accounts to issue a refund to your account. Unfortunately we do not have an antibody to CEBPG available. I would suggest going to the "World's Antibody Gateway" on our website to search 249 other sup...

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Product ab15049 is labeled a monoclonal antibody to CEBPG and the Entrez link provided, (1054 Human), confirms this. Unfortunately, BLAST searching the recognition sequence you have provided (MHRLLAWDAACLPPPAAFPKLH), I find this actually recognizes CE...

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Thank you very much for pointing this out. This antibody, Ab15049, is actually a CEBPB antibody, not CEBPG as listed. I apologize for the error, and this will be corrected on the datasheet. Please do not hesitate to contact us if you need anything...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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