Anti-CEBP Beta antibody [18F8] (ab15049)
![Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)](https://www.abcam.com/ps/products/15/ab15049/Images/ab15049-22280-anti-cebp-beta-antibody-18f8-western-blot.jpg "Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)"){kind=small}
Key features and details
- Mouse monoclonal [18F8] to CEBP Beta
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CEBP Beta antibody [18F8]
See all CEBP Beta primary antibodies -
Description
Mouse monoclonal [18F8] to CEBP Beta -
Host species
Mouse -
Specificity
Ab15049 only detects the LAP* isoform of CEBPG beta. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse CEBP Beta aa 1-19 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:MHRLLAWDAACLPPPPAAF
(Peptide available asab39810) -
Positive control
- Transfected 293T cells, P388D1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
18F8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810).
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810). |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. -
Tissue specificity
Expressed at low levels in the lung, kidney and spleen. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsSumoylated by polymeric chains of SUMO2 or SUMO3. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 12608 Mouse
- SwissProt: P28033 Mouse
- Unigene: 439656 Mouse
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Alternative names
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
see all
Images
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Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)
Western blot of the CEBP Beta using ab15049 on transfected CEBP beta.
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![Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)](https://www.abcam.com/ps/products/15/ab15049/Images/ab15049-22279-anti-cebp-beta-antibody-18f8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)Immunofluorescence staining of CEBP beta in P388D1 cells using ab15049.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab15049 has been referenced in 8 publications.
- Fafián-Labora JA et al. Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage. Cell Metab 32:71-86.e5 (2020). PubMed: 32574561
- Liu SP et al. Secukinumab attenuates reactive astrogliosis via IL-17RA/(C/EBPß)/SIRT1 pathway in a rat model of germinal matrix hemorrhage. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31020769
- Romanel A et al. Inherited determinants of early recurrent somatic mutations in prostate cancer. Nat Commun 8:48 (2017). PubMed: 28663546
- Jang MA et al. a-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS One 12:e0170699 (2017). PubMed: 28114367
- Liu Q et al. Cyclin D1 and C/EBPß LAP1 operate in a common pathway to promote mammary epithelial cell differentiation. Mol Cell Biol 34:3168-79 (2014). PubMed: 24912680
- Atwood AA et al. Negative Regulation of C/EBPbeta1 by Sumoylation in Breast Cancer Cells. PLoS One 6:e25205 (2011). WB ; Human . PubMed: 21980398
- Atwood AA & Sealy L Regulation of C/EBPbeta1 by Ras in mammary epithelial cells and the role of C/EBPbeta1 in oncogene-induced senescence. Oncogene 29:6004-15 (2010). WB ; Human . PubMed: 20818427
- Su WC et al. Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene. J Biol Chem 278:51150-8 (2003). PubMed: 14530280
