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    cebp-beta-antibody-18f8-ab15049.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-CEBP Beta antibody [18F8] (ab15049)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (7)

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Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)
  • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)

Key features and details

  • Mouse monoclonal [18F8] to CEBP Beta
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CEBP Beta antibody [18F8]
    See all CEBP Beta primary antibodies
  • Description

    Mouse monoclonal [18F8] to CEBP Beta
  • Host species

    Mouse
  • Specificity

    Ab15049 only detects the LAP* isoform of CEBPG beta.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse CEBP Beta aa 1-19 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MHRLLAWDAACLPPPPAAF


    (Peptide available as ab39810)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Transfected 293T cells, P388D1 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    18F8
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Immunizing Peptide (Blocking)

    • Mouse CEBP Beta peptide (ab39810)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Application Abreviews Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810).
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810).
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • Tissue specificity

    Expressed at low levels in the lung, kidney and spleen.
  • Sequence similarities

    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P17676 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12608 Mouse
    • SwissProt: P28033 Mouse
    • Unigene: 439656 Mouse
    • Alternative names

      • AGP/EBP antibody
      • C EBP beta antibody
      • C/EBP beta antibody
      • C/EBP related protein 2 antibody
      • CCAAT Enhancer Binding Protein beta antibody
      • CCAAT/enhancer-binding protein beta antibody
      • CEBPB antibody
      • CEBPB_HUMAN antibody
      • CRP2 antibody
      • IL 6DBP antibody
      • IL6DBP antibody
      • Interleukin 6 dependent binding protein antibody
      • LAP antibody
      • Liver activator protein antibody
      • Liver enriched transcriptional activator antibody
      • NF IL6 antibody
      • NFIL6 antibody
      • Nuclear factor NF IL6 antibody
      • Nuclear factor NF-IL6 antibody
      • SF B antibody
      • SFB antibody
      • Silencer factor B antibody
      • TCF-5 antibody
      • TCF5 antibody
      • Transcription factor 5 antibody
      see all

    Images

    • Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)
      Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)

      Western blot of the CEBP Beta using ab15049 on transfected CEBP beta.

    • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)
      Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)
      Immunofluorescence staining of CEBP beta in P388D1 cells using ab15049.

    Protocols

    • Recommended extraction protocol for WB with ab1549

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab15049? Please let us know so that we can cite the reference in this datasheet.

    ab15049 has been referenced in 7 publications.

    • Liu SP  et al. Secukinumab attenuates reactive astrogliosis via IL-17RA/(C/EBPß)/SIRT1 pathway in a rat model of germinal matrix hemorrhage. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31020769
    • Romanel A  et al. Inherited determinants of early recurrent somatic mutations in prostate cancer. Nat Commun 8:48 (2017). PubMed: 28663546
    • Jang MA  et al. a-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS One 12:e0170699 (2017). PubMed: 28114367
    • Liu Q  et al. Cyclin D1 and C/EBPß LAP1 operate in a common pathway to promote mammary epithelial cell differentiation. Mol Cell Biol 34:3168-79 (2014). PubMed: 24912680
    • Atwood AA  et al. Negative Regulation of C/EBPbeta1 by Sumoylation in Breast Cancer Cells. PLoS One 6:e25205 (2011). WB ; Human . PubMed: 21980398
    • Atwood AA & Sealy L Regulation of C/EBPbeta1 by Ras in mammary epithelial cells and the role of C/EBPbeta1 in oncogene-induced senescence. Oncogene 29:6004-15 (2010). WB ; Human . PubMed: 20818427
    • Su WC  et al. Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene. J Biol Chem 278:51150-8 (2003). PubMed: 14530280

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Inquiry: I have C/EBP beta antibody (ab15049). data sheet say that ab15049 only detect the LAP isoform of C/EBP beta. C/EBP beta has three isoforms like FL (full length), LAP and LIP. Sometimes, in western blot, FL and LAP can be hard to be distinguished. So my question is ab15049 detect only LAP or may detect FL also. please let me know

    Read More

    Abcam community

    Verified customer

    Asked on Jul 10 2012

    Answer

    There are three isoforms of C/EBP Beta; LAP*(38kDa), LAP(35kDa) and LIP(20 kDa). Our information specifies that this antibody only detects LAP* or full length as you describe. The immunogen used to make this antibody covers amino acids 1-21 of the full amino acid sequence. LAP and LIP are missing the first 21 and 151 amino acids respectively, so the MA1-826 antibody should not detect them.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 10 2012

    Question

    Unfortunately I have already ordered and paid for this. Do you have a CEBPG antibody, poly or monoclonal which I can order? Can I assume that I will will be reimbursed for my order?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2005

    Answer

    I sincerely apologize for the error. I will ask accounts to issue a refund to your account. Unfortunately we do not have an antibody to CEBPG available. I would suggest going to the "World's Antibody Gateway" on our website to search 249 other suppliers to find the antibody you are looking for. Again, I am very sorry that our datasheet was incorrect, and the datasheet will be changed to the proper information for future reference.

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2005

    Question

    Product ab15049 is labeled a monoclonal antibody to CEBPG and the Entrez link provided, (1054 Human), confirms this. Unfortunately, BLAST searching the recognition sequence you have provided (MHRLLAWDAACLPPPAAFPKLH), I find this actually recognizes CEBPB, Entrez (1051 Human). Futher complicating things I find that this is actually the same recognition sequence you provide for your CEBPB antibody product ab18327. So the question, is this in fact an antibody for CEBPG and the wrong recognition sequence was provided or is this an antibody for CEBPB?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2005

    Answer

    Thank you very much for pointing this out. This antibody, Ab15049, is actually a CEBPB antibody, not CEBPG as listed. I apologize for the error, and this will be corrected on the datasheet. Please do not hesitate to contact us if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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