Anti-CEBP Beta antibody [18F8] (ab15049)

Submit a review Q&A (3)References (5)

Overview

  • Product name
    Anti-CEBP Beta antibody [18F8]
    See all CEBP Beta primary antibodies
  • Description
    Mouse monoclonal [18F8] to CEBP Beta
  • Host species
    Mouse
  • Specificity
    Ab15049 only detects the LAP* isoform of CEBPG beta.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse CEBP Beta aa 1-19 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MHRLLAWDAACLPPPPAAF


    (Peptide available as ab39810)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Transfected 293T cells, P388D1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • Tissue specificity
    Expressed at low levels in the lung, kidney and spleen.
  • Sequence similarities
    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Domain
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AGP/EBP antibody
    • C EBP beta antibody
    • C/EBP beta antibody
    • C/EBP related protein 2 antibody
    • CCAAT Enhancer Binding Protein beta antibody
    • CCAAT/enhancer-binding protein beta antibody
    • CEBPB antibody
    • CEBPB_HUMAN antibody
    • CRP2 antibody
    • IL 6DBP antibody
    • IL6DBP antibody
    • Interleukin 6 dependent binding protein antibody
    • LAP antibody
    • Liver activator protein antibody
    • Liver enriched transcriptional activator antibody
    • NF IL6 antibody
    • NFIL6 antibody
    • Nuclear factor NF IL6 antibody
    • Nuclear factor NF-IL6 antibody
    • SF B antibody
    • SFB antibody
    • Silencer factor B antibody
    • TCF-5 antibody
    • TCF5 antibody
    • Transcription factor 5 antibody
    see all

Images

  • Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)
    Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)

    Western blot of the CEBP Beta using ab15049 on transfected CEBP beta.

  • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)
    Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)
    Immunofluorescence staining of CEBP beta in P388D1 cells using ab15049.

References

This product has been referenced in:
  • Jang MA  et al. a-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS One 12:e0170699 (2017). Read more (PubMed: 28114367) »
  • Liu Q  et al. Cyclin D1 and C/EBPß LAP1 operate in a common pathway to promote mammary epithelial cell differentiation. Mol Cell Biol 34:3168-79 (2014). Read more (PubMed: 24912680) »
See all 5 Publications for this product

Publishing research using ab15049? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Inquiry: I have C/EBP beta antibody (ab15049). data sheet say that ab15049 only detect the LAP isoform of C/EBP beta. C/EBP beta has three isoforms like FL (full length), LAP and LIP. Sometimes, in western blot, FL and LAP can be hard to be distinguished. So my question is ab15049 detect only LAP or may detect FL also. please let me know

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Answer

There are three isoforms of C/EBP Beta; LAP*(38kDa), LAP(35kDa) and LIP(20 kDa). Our information specifies that this antibody only detects LAP* or full length as you describe. The immunogen used to make this antibody covers amino acids 1-21 of the full amino acid sequence. LAP and LIP are missing the first 21 and 151 amino acids respectively, so the MA1-826 antibody should not detect them.

I hope this is helpful. Please contact me again if you have any further questions.

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Question

Unfortunately I have already ordered and paid for this. Do you have a CEBPG antibody, poly or monoclonal which I can order? Can I assume that I will will be reimbursed for my order?

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Answer

I sincerely apologize for the error. I will ask accounts to issue a refund to your account. Unfortunately we do not have an antibody to CEBPG available. I would suggest going to the "World's Antibody Gateway" on our website to search 249 other suppliers to find the antibody you are looking for. Again, I am very sorry that our datasheet was incorrect, and the datasheet will be changed to the proper information for future reference.

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Question

Product ab15049 is labeled a monoclonal antibody to CEBPG and the Entrez link provided, (1054 Human), confirms this. Unfortunately, BLAST searching the recognition sequence you have provided (MHRLLAWDAACLPPPAAFPKLH), I find this actually recognizes CEBPB, Entrez (1051 Human). Futher complicating things I find that this is actually the same recognition sequence you provide for your CEBPB antibody product ab18327. So the question, is this in fact an antibody for CEBPG and the wrong recognition sequence was provided or is this an antibody for CEBPB?

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Answer

Thank you very much for pointing this out. This antibody, Ab15049, is actually a CEBPB antibody, not CEBPG as listed. I apologize for the error, and this will be corrected on the datasheet. Please do not hesitate to contact us if you need anything further.

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