Anti-CEBP Beta antibody [1H7] (ab15050)
Key features and details
- Mouse monoclonal [1H7] to CEBP Beta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CEBP Beta antibody [1H7]
See all CEBP Beta primary antibodies -
Description
Mouse monoclonal [1H7] to CEBP Beta -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length LIP protein (Mouse)
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Positive control
- ICC/IF: P388D1 cells WB: 3T3-L1 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1H7 -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 4 µg/ml. Fix with 4% paraformaldehyde.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35,38,20 kDa).
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Notes |
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ICC/IF
Use a concentration of 4 µg/ml. Fix with 4% paraformaldehyde. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35,38,20 kDa). |
Target
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Function
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. -
Tissue specificity
Expressed at low levels in the lung, kidney and spleen. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsSumoylated by polymeric chains of SUMO2 or SUMO3. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 12608 Mouse
- SwissProt: P28033 Mouse
- Unigene: 439656 Mouse
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Alternative names
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab15050 has been referenced in 14 publications.
- Liu M et al. PERK reprograms hematopoietic progenitor cells to direct tumor-promoting myelopoiesis in the spleen. J Exp Med 219:N/A (2022). PubMed: 35266960
- Zhu Q et al. Protein arginine methyltransferase PRMT1 promotes adipogenesis by modulating transcription factors C/EBPβ and PPARγ. J Biol Chem 298:102309 (2022). PubMed: 35921899
- Li M et al. Regulation of MYB by distal enhancer elements in human myeloid leukemia. Cell Death Dis 12:223 (2021). PubMed: 33637692
- Gao J et al. LncRNA LEF1-AS1 silencing diminishes EZH2 expression to delay hepatocellular carcinoma development by impairing CEBPB-interaction with CDCA7. Cell Cycle 19:870-883 (2020). PubMed: 32178558
- Bastola S et al. Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy. Nat Commun 11:4660 (2020). PubMed: 32938908