Product nameAnti-CEBP Beta (phospho T235 + T188) antibody
See all CEBP Beta primary antibodies
DescriptionRabbit polyclonal to CEBP Beta (phospho T235 + T188)
SpecificityThis antibody detects endogenous levels of CEBP Beta only when phosphorylated at threonine 235/188.
Tested applicationsSuitable for: ICC/IF, IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic phosphopeptide derived from human CEBP Beta around the phosphorylation site of threonine 235/188.
- Human breast carcinoma tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab52194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. PubMed: 22144112|
FunctionImportant transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
Tissue specificityExpressed at low levels in the lung, kidney and spleen.
Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
modificationsSumoylated by polymeric chains of SUMO2 or SUMO3.
- Information by UniProt
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
ab52194 staining CEBP Beta (phospho T335 + T188) in Human MDA-MB-468 breast cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde + 2% sucrose and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (in PBS + 1% BSA) for 30 minutes at 37°C.
From left - DAPI (blue), MUC1-C (green), CERP Beta phospho T335 + T188 (red), Merge, Zoom.
Top - CshRNA, Bottom - MUC1shRNA.
Magnification - x63, Zoom (from pink boxes) - x252.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab52194 at a 1/50 dilution.
Left image un-treated.
Right image treated with phosphopeptide.
This product has been referenced in:
- Alam M et al. MUC1-C Oncoprotein Activates ERK->C/EBPß Signaling and Induction of Aldehyde Dehydrogenase 1A1 in Breast Cancer Cells. J Biol Chem 288:30892-903 (2013). ICC/IF ; Human . Read more (PubMed: 24043631) »
- Chen CJ et al. Glutamate released by Japanese encephalitis virus-infected microglia involves TNF-a signaling and contributes to neuronal death. Glia 60:487-501 (2012). WB . Read more (PubMed: 22144112) »