• Product name

    Anti-CEBP Beta (phospho T235 + T188) antibody
    See all CEBP Beta primary antibodies
  • Description

    Rabbit polyclonal to CEBP Beta (phospho T235 + T188)
  • Host species

  • Specificity

    This antibody detects endogenous levels of CEBP Beta only when phosphorylated at threonine 235/188.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human CEBP Beta around the phosphorylation site of threonine 235/188.

  • Positive control

    • Human breast carcinoma tissue



Our Abpromise guarantee covers the use of ab52194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB Use at an assay dependent concentration. PubMed: 22144112


  • Function

    Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • Tissue specificity

    Expressed at low levels in the lung, kidney and spleen.
  • Sequence similarities

    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational

    Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • AGP/EBP antibody
    • C EBP beta antibody
    • C/EBP beta antibody
    • C/EBP related protein 2 antibody
    • CCAAT Enhancer Binding Protein beta antibody
    • CCAAT/enhancer-binding protein beta antibody
    • CEBPB antibody
    • CEBPB_HUMAN antibody
    • CRP2 antibody
    • IL 6DBP antibody
    • IL6DBP antibody
    • Interleukin 6 dependent binding protein antibody
    • LAP antibody
    • Liver activator protein antibody
    • Liver enriched transcriptional activator antibody
    • NF IL6 antibody
    • NFIL6 antibody
    • Nuclear factor NF IL6 antibody
    • Nuclear factor NF-IL6 antibody
    • SF B antibody
    • SFB antibody
    • Silencer factor B antibody
    • TCF-5 antibody
    • TCF5 antibody
    • Transcription factor 5 antibody
    see all


  • ab52194 staining CEBP Beta (phospho T335 + T188) in Human MDA-MB-468 breast cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde + 2% sucrose and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (in PBS + 1% BSA) for 30 minutes at 37°C.

    From left - DAPI (blue), MUC1-C (green), CERP Beta phospho T335 + T188 (red), Merge, Zoom.

    Top - CshRNA, Bottom - MUC1shRNA.

    Magnification - x63, Zoom (from pink boxes) - x252.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab52194 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.


This product has been referenced in:

  • Li CC  et al. 14-Deoxy-11,12-didehydroandrographolide suppresses adipogenesis of 3 T3-L1 preadipocytes by inhibiting CCAAT/enhancer-binding protein ß activation and AMPK-mediated mitotic clonal expansion. Toxicol Appl Pharmacol 359:82-90 (2018). Read more (PubMed: 30248417) »
  • Alam M  et al. MUC1-C Oncoprotein Activates ERK->C/EBPß Signaling and Induction of Aldehyde Dehydrogenase 1A1 in Breast Cancer Cells. J Biol Chem 288:30892-903 (2013). ICC/IF ; Human . Read more (PubMed: 24043631) »
See all 4 Publications for this product

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