Anti-CEBPG antibody (ab74045)
Key features and details
- Rabbit polyclonal to CEBPG
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CEBPG antibody -
Description
Rabbit polyclonal to CEBPG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human CEBPG (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1054 Human
- Entrez Gene: 12611 Mouse
- Entrez Gene: 25301 Rat
- Omim: 138972 Human
- SwissProt: P53567 Human
- SwissProt: P53568 Mouse
- SwissProt: P26801 Rat
- Unigene: 429666 Human
see all -
Alternative names
- C/EBP gamma antibody
- CCAAT/enhancer binding protein (C/EBP) gamma antibody
- CCAAT/enhancer binding protein gamma antibody
see all
Images
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All lanes : Anti-CEBPG antibody (ab74045) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
Lane 2 : Extracts from RAW264.7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 16 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab74045, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab74045 has been referenced in 3 publications.
- Ebert SM et al. Activating transcription factor 4 (ATF4) promotes skeletal muscle atrophy by forming a heterodimer with the transcriptional regulator C/EBPß. J Biol Chem 295:2787-2803 (2020). PubMed: 31953319
- Sun X et al. Dominant-Negative ATF5 Compromises Cancer Cell Survival by Targeting CEBPB and CEBPD. Mol Cancer Res 18:216-228 (2020). PubMed: 31676720
- Zeng S et al. ETV4 collaborates with Wnt/ß-catenin signaling to alter cell cycle activity and promote tumor aggressiveness in gastrointestinal stromal tumor. Oncotarget 8:114195-114209 (2017). PubMed: 29371979