Recombinant
RabMAb

Recombinant Anti-CEECAM1 antibody [EPR13744] (ab181979)

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Overview

  • Product name

    Anti-CEECAM1 antibody [EPR13744]
    See all CEECAM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13744] to CEECAM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CEECAM1 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q5T4B2

  • Positive control

    • Human fetal brain, Jurkat, 293, U87-MG lysates; 293 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13744

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Flow Cyt 1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Western blot - Anti-CEECAM1 antibody [EPR13744] (ab181979)
    Western blot - Anti-CEECAM1 antibody [EPR13744] (ab181979)
    All lanes : Anti-CEECAM1 antibody [EPR13744] (ab181979) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

  • Flow Cytometry - Anti-CEECAM1 antibody [EPR13744] (ab181979)
    Flow Cytometry - Anti-CEECAM1 antibody [EPR13744] (ab181979)

    Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling CEECAM1 with ab181979 at 1/300 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab181979 has not yet been referenced specifically in any publications.

Publishing research using ab181979? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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