Overview

  • Product name

    Anti-CELF-6 antibody [EPR10155]
    See all CELF-6 primary antibodies
  • Description

    Rabbit monoclonal [EPR10155] to CELF-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CELF-6 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96J87
    (Peptide available as ab202757)

  • Positive control

    • Human fetal kidney and fetal brain lysates; HepG2 and 293T cell lysates; Human kidney tissue; 29T cells.
  • General notes

    Previously labelled as BRUNOL6.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.

WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.Can be blocked with CELF-6 peptide (ab202757).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in a muscle-specific splicing enhancer (MSE)-dependent manner. Promotes also exon exclusion of INSR pre-mRNA.
    • Tissue specificity

      Expressed mainly in kidney, brain and testis and present in other tissues albeit at lower levels. Also expressed in fetal kidney.
    • Sequence similarities

      Belongs to the CELF/BRUNOL family.
      Contains 3 RRM (RNA recognition motif) domains.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 6330569O16Rik antibody
      • Bruno like 6, RNA binding protein (Drosophila) antibody
      • bruno like 6, RNA binding protein antibody
      • Bruno like protein 6 antibody
      • Bruno-like protein 6 antibody
      • BRUNOL6 antibody
      • CELF-6 antibody
      • Celf6 antibody
      • CELF6_HUMAN antibody
      • CUG-BP- and ETR-3-like factor 6 antibody
      • CUGBP and ETR3 like factor 6 antibody
      • CUGBP Elav-like family member 6 antibody
      • RNA binding protein BRUNOL 6 antibody
      • RNA-binding protein BRUNOL-6 antibody
      see all

    Images

    • Flow cytometry analysis of permeabilized 293T cells labeling CELF-6 (red), using ab173282 at a 1/10 dilution, or negative control rabbit IgG (green)

    • All lanes : Anti-CELF-6 antibody [EPR10155] (ab173282) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 50 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling CELF-6 using ab173282 at a 1/50 dilution.

    References

    ab173282 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173282.
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