Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10155] to CELF-6
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-CELF-6 antibody [EPR10155]
See all CELF-6 primary antibodies
DescriptionRabbit monoclonal [EPR10155] to CELF-6
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
- Human fetal kidney and fetal brain lysates; HepG2 and 293T cell lysates; Human kidney tissue; 29T cells.
Previously labelled as BRUNOL6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab173282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended.
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.Can be blocked with CELF-6 peptide (ab202757).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in a muscle-specific splicing enhancer (MSE)-dependent manner. Promotes also exon exclusion of INSR pre-mRNA.
Tissue specificityExpressed mainly in kidney, brain and testis and present in other tissues albeit at lower levels. Also expressed in fetal kidney.
Sequence similaritiesBelongs to the CELF/BRUNOL family.
Contains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- 6330569O16Rik antibody
- Bruno like 6, RNA binding protein (Drosophila) antibody
- bruno like 6, RNA binding protein antibody
Flow cytometry analysis of permeabilized 293T cells labeling CELF-6 (red), using ab173282 at a 1/10 dilution, or negative control rabbit IgG (green)
All lanes : Anti-CELF-6 antibody [EPR10155] (ab173282) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling CELF-6 using ab173282 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab173282 has been referenced in 1 publication.
- Liu G et al. RNA-binding protein CELF6 is cell cycle regulated and controls cancer cell proliferation by stabilizing p21. Cell Death Dis 10:688 (2019). PubMed: 31534127