Overview

  • Product name

    Cell Counting Kit 8 (WST-8 / CCK8)
    See all Cell viability/proliferation kits
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells
  • Product overview

    Cell Counting Kit 8 (WST-8 / CCK8) (ab228554) provides a convenient and robust way of performing a cell viability assay. The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier.


    WST-8 / CCK8 solution is added directly to the test cells with no pre-mixing of components required.


    WST-8 / CCK8 tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells and is measured by absorbance at 460 nm.


    The excellent stability and little cytotoxicity of the WST-8 / CCK8 solution make the kit useful for assays that require long incubation (such as 24 to 48 hours).


    The detection sensitivity is higher than with other tetrazolium salt-based assays such as MTT, XTT or MTS etc.

  • Notes

    Review the cell health assay guide to learn about other kits to perform a cell viability assaycytotoxicity assay or cell proliferation assay.

  • Platform

    Microplate reader

Properties

Images

  • Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with The cell Counting Kit 8 (WST-8) (ab228554). HeLa cells at 10,000 cells/well/100 μL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a plate reader.

  • Cell number was determined with the cell Counting Kit 8 (WST-8) (ab228554). (A) HeLa cells at 0 to 10,000 cells/well/100 μL, and (B) Jurkat cells at 0 to 100,000 cells/well/100 μL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a plate reader.

Protocols

References

This product has been referenced in:

  • Lyu X  et al. LncRNA NKILA Inhibits Retinoblastoma by Downregulating lncRNA XIST. Curr Eye Res N/A:1-5 (2019). Read more (PubMed: 30995132) »
  • Wu XY  et al. BF211, a derivative of bufalin, enhances the cytocidal effects in multiple myeloma cells by inhibiting the IL-6/JAK2/STAT3 pathway. Int Immunopharmacol 64:24-32 (2018). Read more (PubMed: 30145467) »
See all 2 Publications for this product

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