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Explore the power of knock-out cell lines for your research

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    cell-counting-kit-8-wst-8--cck8-ab228554.pdf

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Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)

  • Datasheet
  • Protocol Booklet
Reviews (2) Submit a question References (65)

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Cytotoxicity tests of HeLa cells.
  • Cell Counting Assay.

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Cell Counting Kit 8 (WST-8 / CCK8)
    See all Cell viability/proliferation kits
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells
  • Product overview

    Cell Counting Kit 8 (WST-8 / CCK8) (ab228554) provides a convenient and robust way of performing a cell viability assay. The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier.


    WST-8 / CCK8 solution is added directly to the test cells with no pre-mixing of components required.


    WST-8 / CCK8 tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells and is measured by absorbance at 460 nm.


    The excellent stability and little cytotoxicity of the WST-8 / CCK8 solution make the kit useful for assays that require long incubation (such as 24 to 48 hours).


    The detection sensitivity is higher than with other tetrazolium salt-based assays such as MTT, XTT or MTS etc.

  • Notes

    Review the cell health assay guide to learn about other kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1000 tests
    WST-8 Solution 1 x 10ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
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    • Cell Viability and Senescence Kits
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    • Kits
    • Cell Damage Kits
    • proliferation and senescence
  • Relevance

    Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
  • Alternative names

    • Cell Cytotoxicity
    • cell tracking

Images

  • Cytotoxicity tests of HeLa cells.
    Cytotoxicity tests of HeLa cells.

    Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with The cell Counting Kit 8 (WST-8) (ab228554). HeLa cells at 10,000 cells/well/100 μL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a plate reader.

  • Cell Counting Assay.
    Cell Counting Assay.

    Cell number was determined with the cell Counting Kit 8 (WST-8) (ab228554). (A) HeLa cells at 0 to 10,000 cells/well/100 μL, and (B) Jurkat cells at 0 to 100,000 cells/well/100 μL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a plate reader.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (65)

Publishing research using ab228554? Please let us know so that we can cite the reference in this datasheet.

ab228554 has been referenced in 65 publications.

  • Li S  et al. NLRX1/FUNDC1/NIPSNAP1-2 axis regulates mitophagy and alleviates intestinal ischaemia/reperfusion injury. Cell Prolif 54:e12986 (2021). PubMed: 33432610
  • Liang Q  et al. Osteopontin-induced lncRNA HOTAIR expression is involved in osteoarthritis by regulating cell proliferation. BMC Geriatr 21:57 (2021). PubMed: 33446111
  • Fan K  et al. Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without glucocorticoid side effect. Theranostics 11:2670-2690 (2021). PubMed: 33456566
  • Zhang Y  et al. Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs. Genome Biol 22:41 (2021). PubMed: 33478577
  • Huang L  et al. Comparative Study on 3D Printed Ti6Al4V Scaffolds with Surface Modifications Using Hydrothermal Treatment and Microarc Oxidation to Enhance Osteogenic Activity. ACS Omega 6:1465-1476 (2021). PubMed: 33490806
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Abreview for ab228554 - Cell Counting Kit 8 (WST-8 / CCK8)

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
4000 cells per well were cultured with 10% serum medium and relevant pharmacological compounds. Cell proliferation was detected by using this ab228554 - Cell Counting Kit 8 (WST-8 / CCK8) according to the manufacturer's instructions, the final optical density from all of samples were between the lowest and the highest standard.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 28 2022

easiest cell viability assay I've ever played with

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
works great with my cell line, easy to use. 4 hours of incubation was performed in order to increase sensitivity for cells fewer than 10k.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 17 2022

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