Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)
Key features and details
- Detection method: Fluorescent
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Cycle Assay Kit (Fluorometric - Green)
See all Cell Cycle kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Green CCS1 can be monitored with a flow cytometer at Ex/Em = 490 nm/520 nm (FL1 channel).
Visit our FAQs page for tips and troubleshooting.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 50ml Nuclear Green CCS1 1 x 250µl -
Research areas
Images
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Histogram of HeLa cells (black: untreated cells, red : apoptotic cells) stained with 0.1x Nuclear Green CCS1
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DNA profile in growing and camptothecin treated Jurkat cells.
Jurkat cells were treated without (red) or with 20 µM camptothecin (blue) in a 37°C, 5% CO2 incubator for ~8 hours, then dye loaded with 200X Green Fluorescence Probe for 60 minutes. The fluorescence intensity of 200X Green Fluorescence Probe was measured with a flow cytometer using the FL1 channel.
Datasheets and documents
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Datasheet download
References (13)
ab112116 has been referenced in 13 publications.
- Li H et al. Pepsin enhances glycolysis to promote malignant transformation of vocal fold leukoplakia epithelial cells with dysplasia. Eur Arch Otorhinolaryngol 280:1841-1854 (2023). PubMed: 36380093
- Huang YG et al. CENPF/CDK1 signaling pathway enhances the progression of adrenocortical carcinoma by regulating the G2/M-phase cell cycle. J Transl Med 20:78 (2022). PubMed: 35123514
- Lin H et al. COA3 overexpression promotes non-small cell lung cancer metastasis by reprogramming glucose metabolism. Am J Cancer Res 12:3662-3678 (2022). PubMed: 36119836
- Li B et al. Characterizing the Low-Dose Effects of Methylmercury on the Early Stages of Embryo Development Using Cultured Human Embryonic Stem Cells. Environ Health Perspect 129:77007 (2021). PubMed: 34328791
- Huang YG et al. EMS1/DLL4-Notch Signaling Axis Augments Cell Cycle-Mediated Tumorigenesis and Progress in Human Adrenocortical Carcinoma. Front Oncol 11:771579 (2021). PubMed: 34858850