Overview

  • Product name
    Cell Cycle Assay Kit (Fluorometric - Green)
    See all Cell Cycle kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Green CCS1 can be monitored with a flow cytometer at Ex/Em = 490 nm/520 nm (FL1 channel).

    Visit our FAQs page for tips and troubleshooting.

  • Tested applications
    Suitable for: Flow Cytmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab112116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Images

  • Histogram of HeLa cells (black: untreated cells, red : apoptotic cells) stained with 0.1x Nuclear Green CCS1

  • DNA profile in growing and camptothecin treated Jurkat cells.

    Jurkat cells were treated without (red) or with 20 µM camptothecin (blue) in a 37°C, 5% CO2 incubator for ~8 hours, then dye loaded with 200X Green Fluorescence Probe for 60 minutes. The fluorescence intensity of 200X Green Fluorescence Probe was measured with a flow cytometer using the FL1 channel.

Protocols

References

This product has been referenced in:
  • Yuen JWM  et al. Tumor inhibitory effects of intravesical Ganoderma lucidum instillation in the syngeneic orthotopic MB49/C57 bladder cancer mice model. J Ethnopharmacol 223:113-121 (2018). Mouse . Read more (PubMed: 29783018) »
  • Sun X & Zhang L MicroRNA-143 suppresses oral squamous cell carcinoma cell growth, invasion and glucose metabolism through targeting hexokinase 2. Biosci Rep 37:N/A (2017). Human . Read more (PubMed: 28174335) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Yes, you are right. The two distinct peaks are easy to see G1 and G2, the S phase is between G1 and G2 and it is not peak but more like a flat population, it is much easier to see it if you plot it as linear instead of log on X axis. See the following links and attached document for further details.


http://www.ucl.ac.uk/wibr/services/docs/cellcyc.pdf

http://www.horizonpress.com/cimb/v/v3/v3n303.pdf

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Answer

Yes, these two kits are not species specific.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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