Overview

  • Product name
    Cell Cycle Assay Kit (Fluorometric - Red)
    See all Cell Cycle kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (quantitative)
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Store ab112117 desiccated.

  • Platform
    Flow cytometer

Properties

Images

  • DNA profile in growing Jurkat cells.
    Jurkat cells were dye-loaded with Red Fluorescence Probe and RNase A for 30 minutes. The fluorescence intensity of Red Fluorescence Probe was measured with a flow cytometer, using the FL2 channel.

Protocols

References

This product has been referenced in:
  • Parada CA  et al. Kinome and phosphoproteome of high-grade meningiomas reveal AKAP12 as a central regulator of aggressiveness and its possible role in progression. Sci Rep 8:2098 (2018). Functional Studies . Read more (PubMed: 29391485) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Yes, these two kits are not species specific.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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