Overview

  • Product name

    Cell Cytotoxicity Assay Kit (Colorimetric)
    See all Cell Cytotoxicity kits
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. There are a variety of parameters that can be used. ab112118 uses a proprietary water-soluble dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make ab112118 suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.


    ab112118 does not require pre-mixing of components and has higher sensitivity compared to tetrazolium based colorimetric assays (such as MTT and XTT). It comes with reagents sufficient to run 1000 assays. The kit components are quite stable with minimal cytotoxicity, thus longer incubation times (such as 24-48 hours) are possible if required.
    ab112118 is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

    Visit our FAQs page for tips and troubleshooting.


    Cytotoxicity assay protocol summary:
    - add assay solution to cells and shake for 30 s
    - incubate for 1-24 hrs
    - analyze with microplate reader

  • Notes

    ab112118 should be stored desiccated.

    As low as 300 cells can be accurately quantified using ab112118.

  • Platform

    Microplate reader

Properties

Images

  • •HeLa: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.

    •NIH3T3: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.

  • CHO-K1 cell number response was measured with ab112118. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 oC. The absorbance intensity was measured at 570 nm and 605 nm. The ratio of OD570/OD605 is proportional to the number of cells as indicated.

Protocols

References

This product has been referenced in:

  • Ayyash M  et al. In vitro investigation of bioactivities of solid-state fermented lupin, quinoa and wheat using Lactobacillus spp. Food Chem 275:50-58 (2019). Read more (PubMed: 30724226) »
  • Lautenschläger J  et al. C-terminal calcium binding of a-synuclein modulates synaptic vesicle interaction. Nat Commun 9:712 (2018). Read more (PubMed: 29459792) »
See all 6 Publications for this product

Customer reviews and Q&As

Question
Answer


ab112118 is compatible with media containing phenol red. Any potential background will be subtracted in your background wells. DTT should not be present as it interferes with the assay. For the background control wells (media without cells), please use fresh media as the dye is pH sensitive, if the media is several days old, the pH difference will give different readings with cells vs. media only.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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