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Will phenol red in media interfere with the assay ab112118?
Asked on Nov 17 2016
ab112118 is compatible with media containing phenol red. Any potential background will be subtracted in your background wells. DTT should not be present as it interferes with the assay. For the background control wells (media without cells), please use fresh media as the dye is pH sensitive, if the media is several days old, the pH difference will give different readings with cells vs. media only.
Answered on Nov 17 2016