Effects of dopamine of cell viability

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Increased concentrations of dopamine reduce cell survival independently of α-synuclein presence.
Normal SH-SY5Y cells and SH-SY5Y cells transfected with 1 µg α-synuclein siRNA were treated with varying concentrations of dopamine with and without MG-132 proteasome inhibitor for 72 h. Cell viability was measured using ab112118 cell cytotoxicity assay kit (abcam, Cambridge, UK). Data was analysed using one-way ANOVA corrected by post-hoc Sheffé's test (* p < 0.05, ** p < 0.01) and expressed as a relative percentage of survival compared to PBS vehicle control. Error bars represent ± standard deviation of four independent replicates.

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Submitted Sep 03 2014

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