Key features and details
- Assay type: Direct
- Assay time: 1 hr
Product nameCell Fractionation Kit - High Throughput (HT)
See all Cell Fractionation kits
Assay time1h 00m
Species reactivityReacts with: Mammals, Other species
Abcam's Cell Fractionation Kit - High Throughput (HT), provides a method and reagents for a simple and rapid preparation of cytosolic, mitochondrial and nuclear fractions from adherent cells.
The Cell Fractionation Kit is designed to produce highly enriched fractions that allow for the monitoring of proteins of interest through various cellular compartments. It is not designed to produce purified cellular fractions.
The kit is especially suitable for, but not limited to, high throughput fractionation of adherent cells in a 96-well plate format. The entire fractionation of 96 samples into the cytosolic, mitochondrial and nuclear fraction is achieved in one hour. The kit is based on sequential detergent extraction of cytosolic, mitochondrial and nuclear proteins without the need for mechanical disruption of cells, and thus fractionates adherent cells into cytosol-containing, mitochondria-containing and nuclei-containing fractions. These fractions are referred throughout the protocol as cytosolic, mitochondrial and nuclear fractions. The kit prepares sufficient sample material for subsequent Western blot analyses, dot blot analyses or for analyses by microplate ELISA assays.
ab109718 is designed to allow the measurement of any proteins which are differentially represented in the cytosol, mitochondria and nuclei, and is particularly applicable to studies of proteins that translocate between these three cellular compartments.
A second version of the kit is also available, ab109719 Cell Fractionation Kit - Standard. This version is designed for fractionating cells grown in batches and is ideal for experiments requiring larger sample amounts.
Storage: Store Buffer A, Detergent HT II, Detergent HT III and 5X SDS Sample Buffer at -20°C. Store Detergent HT I at -80°C. Store the collection plates and seal films at room temperature.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 5X SDS Sample Buffer 1 x 1.5ml 96-well collection Plate 3 units Buffer A 1 x 34ml Detergent HT I 1 x 7.5µl Detergent HT II 1 x 35µl Detergent HT III 1 x 700µl Plate Seals 3 units
Measurement and distribution of proteins using Western blot, which are differentially represented in the cytosol, mitochondria and nuclei after treatment with varying concentrations of staurosporine.
Measurement and distribution of proteins using an ELISA, which are differentially represented in the cytosol, mitochondria and nuclei after treatment with varying concentrations of staurosporine.
Cell Fractionation Kit - HT (ab109718).
ab109718 has been referenced in 4 publications.
- Huehnchen P et al. Blockade of IL-6 signaling prevents paclitaxel-induced neuropathy in C57Bl/6 mice. Cell Death Dis 11:45 (2020). PubMed: 31969555
- Lian J et al. Hepatitis B virus upregulates cellular inhibitor of apoptosis protein 2 expression via the PI3K/AKT/NF-?B signaling pathway in liver cancer. Oncol Lett 19:2043-2052 (2020). PubMed: 32194701
- Liang J et al. Myristoylation confers noncanonical AMPK functions in autophagy selectivity and mitochondrial surveillance. Nat Commun 6:7926 (2015). PubMed: 26272043
- García-Gómez S et al. PrimPol, an archaic primase/polymerase operating in human cells. Mol Cell 52:541-53 (2013). PubMed: 24207056