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This kit appears to allow fractionation of mitochondrial proteins from total cellular proteins. Can I use this kit to purify whole mitochondria from the rest of the cell?
Asked on Nov 28 2011
This is quite a common question. The fractionation kit is good for easily and rapidly separating cytosol, Mitochondria and nuclei. This is a great method for making fractions to follow movement of proteins between these compartments such, but not limited to, in apoptosis. To purify Mitochondria and subsequently purify its compartments I would recommend starting with our mitochondria isolation kit ab110169. I hope this helps. Please contact me again if you have any further questions.
Answered on Nov 28 2011