For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Can ab110170 (MS852) be used to isolate cytosolic fractions aswell as Mitochondira?
Asked on Jan 04 2012
Thank you for calling Abcam earlier. For the Mitochondria Isolation Kit for Cultured Cells (ab110170), if you want to isolate the cytosolic fraction, you could keep the supernatant after 'SPIN 2' (see attached protocol) and that would give you a crude fraction. However there is no guarantee that this will give you clean preps without damaging the RNA. I would however recommend ab109718, the Cell Fractionation Kit as this will give you cleaner fractions of the mitochondria and cytosol. Also in the protocol for that product, it gives you the volumes to use for a 24 well plate and you get enough reagents for 1x96 well plate. Please let me know if there is anything else I can help you with.
Answered on Jan 04 2012