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From the protocol and datasheet on your website, it is not clear to me in which fractions plasma membranes, membrane associated proteins and the other cytosolic organels are extracted. Could I have more detailed informations about this?
Asked on Apr 24 2013
We have not tested localization of plasma membrane proteins or cytoplasmic organelles (besides mitochondria) with ab109718. However, we have tested distribution of these compartments using ab139409, a kit which works on the same principal as ab109718. Using ab109718 we found that plasma membrane, ER and golgi proteins are found mostly in the mitochondrial/membrane fraction. For blot images using ab139409 kit on selected targets, please see datasheets below:
If you plan to use ab109718 to fractionate cells into nuclear fraction vs everything else, I would recommend just to skip the extraction with the Detergent HT1.
Answered on Apr 25 2013