Cell Invasion Assay (Basement Membrane), 24-well, 8 µm (ab235882)

Overview

  • Product name

    Cell Invasion Assay (Basement Membrane), 24-well, 8 µm
    See all Cell Invasion kits
  • Detection method

    Fluorescent
  • Sample type

    Suspension cells
  • Species reactivity

    Reacts with: Mouse, Human
  • Product overview

    This kit provides enough materials for 12 assays, i.e. to run 12 wells of assay, although it comes in a 24 well plate format.


    Cell Invasion Assay (Basement Membrane), 24-well, 8 µm (ab235882) utilizes a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.

  • Notes

    Cell Invasion Assay (Basement Membrane), 24-well, 8 µm (ab235882) utilizes a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 12 tests
    Basement Membrane Solution 1 x 1.2ml
    Cell Dissociation Solution   1 x 6ml
    Cell Dye 1 x 1.5ml
    Cell Invasion Chamber  1 unit
    Control Invasion Inducer  1 x 300µl
    Wash Buffer   1 x 25ml

Images

  • HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Invasion Dye and fluorescence (Em/Ex 530/590) was measured.

  • 3T3-NIH and HT-1080 cells were starved overnight and treated with Control Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compared to 3T3-NIH control cells.

Protocols

References

ab235882 has not yet been referenced specifically in any publications.

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